Slominska E M, Adamski P, Lipinski M, Swierczynski J, Smolenski R T
Department of Biochemistry, Medical Univerity of Gdansk, Gdansk, Poland.
Nucleosides Nucleotides Nucleic Acids. 2006;25(9-11):1245-9. doi: 10.1080/15257770600894725.
Monitoring level of the metabolites of the coenzyme NAD such as nicotinamide and its oxidized and methylated derivatives is important due to therapeutic applications of these compounds and monitoring of oxidative stress. We evaluated feasibility of using HPLC with electrospray ion-trap mass detection for single run separation and quantitation of all the NAD metabolites. We achieved good separation and retention of all the metabolites of interest using reversed-phase with ion-pairing. Single ion monitoring or tandem MS were used for detection and quantitation of the specific compounds with good linearity. The method was able to detect all the physiological metabolites in plasma samples of rats and humans or in urine. However, full validation is necessary before this method could be routinely applied.
监测辅酶NAD的代谢产物水平，如烟酰胺及其氧化和甲基化衍生物，由于这些化合物的治疗应用以及氧化应激监测而显得重要。我们评估了使用高效液相色谱与电喷雾离子阱质谱检测法对所有NAD代谢产物进行单次运行分离和定量的可行性。我们通过反相离子对法实现了所有目标代谢产物的良好分离和保留。使用单离子监测或串联质谱对特定化合物进行检测和定量，线性良好。该方法能够检测大鼠和人类血浆样本或尿液中的所有生理代谢产物。然而，在该方法能够常规应用之前，还需要进行全面验证。
