Unge T, Ahola H, Bhikhabhai R, Bäckbro K, Lövgren S, Fenyö E M, Honigman A, Panet A, Gronowitz J S, Strandberg B
Department of Molecular Biology, Uppsala University, Sweden.
AIDS Res Hum Retroviruses. 1990 Nov;6(11):1297-303. doi: 10.1089/aid.1990.6.1297.
The HIV-1 pol gene proteins (protease, reverse transcriptase, and endonuclease) were expressed in Escherichia coli N4830-1 by the use of the inducible expression vector pWS60 into which the pol gene was inserted. The p66/p51 heterodimer of reverse transcriptase (RT) was isolated in a highly pure and active form. Crystals of the p66/p51 heterodimer were obtained by the vapor diffusion hanging drop technique. The present crystal quality is still not adequate for high resolution X-ray investigation.
通过使用插入了 pol 基因的可诱导表达载体 pWS60，在大肠杆菌 N4830-1 中表达了 HIV-1 pol 基因蛋白（蛋白酶、逆转录酶和核酸内切酶）。以高纯度和活性形式分离出了逆转录酶（RT）的 p66/p51 异二聚体。通过气相扩散悬滴技术获得了 p66/p51 异二聚体的晶体。目前的晶体质量仍不足以进行高分辨率 X 射线研究。
