Sloan K E, Stevenson J A, Bigbee J W
University of Miami School of Medicine, FL 33136.
Cell Tissue Res. 1991 Feb;263(2):265-70. doi: 10.1007/BF00318768.
The distribution of phosphorylated and non-phosphorylated neurofilament epitopes was determined immunocytochemically in adjacent 2 microns-thick sections of sciatic nerve, ventral root and spinal cord. Staining was scored as either intense, moderate or absent and the proportion of labeled axons was calculated for each category. Nearly all sciatic nerve and ventral root axons were immunoreactive with both antibodies against phosphorylated and non-phosphorylated neurofilaments and there were no significant differences in the number of intensely- or moderately-labeled axons. Within the spinal cord however, while the majority of large caliber axons was stained with both antibodies, there was a significant number of small caliber axons which stained only with antibodies against phosphorylated neurofilaments. These results show that phosphorylated and non-phosphorylated neurofilaments are extensively codistributed in CNS and PNS axons, and that in the CNS, staining intensity for non-phosphorylated epitopes is less in the smaller axons.
采用免疫细胞化学方法，在坐骨神经、腹根和脊髓相邻的2微米厚切片中，测定磷酸化和非磷酸化神经丝表位的分布。染色结果分为强、中、无三种，并计算每类标记轴突的比例。几乎所有坐骨神经和腹根轴突对磷酸化和非磷酸化神经丝的抗体均有免疫反应，强标记或中度标记轴突的数量无显著差异。然而在脊髓内，虽然大多数大口径轴突对两种抗体均有染色，但有相当数量的小口径轴突仅对磷酸化神经丝抗体有染色。这些结果表明，磷酸化和非磷酸化神经丝在中枢神经系统和外周神经系统轴突中广泛共分布，且在中枢神经系统中，较小轴突中非磷酸化表位的染色强度较低。
