Yu Bing-zhi, Fu Wei, Su Wen-hui, Yu Da-hai, Zhang Zhe, Feng Chen
Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Liaoning Province, PR China.
Cell Biochem Funct. 2007 Nov-Dec;25(6):619-24. doi: 10.1002/cbf.1357.
Effects of PKC zeta on the activation of embryonic transcription in 1-cell stage fertilized mouse eggs were explored. The effects of PKC antagonist calphostin C and PKC zeta specific inhibitor on the activation of embryonic early transcription were observed by Western blotting and cell immunofluorescence. PKC activity increased gradually from G1 phase to late G2 phase in mouse 1-cell stage fertilized eggs, and reached a maximum in G2 stage. Calphostin C inhibited PKC activity by about 47% in 1-cell stage fertilized eggs. Calphostin C inhibited early transcription in 1-cell stage fertilized eggs (p < 0.01). PKC zeta-Thr410 in G2 were about 27% and 110% higher than those in G1 phase of 1-cell stage fertilized eggs and MII oocytes, respectively. PKC zeta specific inhibitor can also inhibit early transcription in 1-cell stage fertilized eggs (p < 0.05). The results suggest that PKC zeta participates in early transcription activation in mouse 1-cell stage fertilized eggs.
研究了蛋白激酶Cζ（PKCζ）对单细胞期受精小鼠卵母细胞胚胎转录激活的影响。通过蛋白质免疫印迹法和细胞免疫荧光法观察了PKC拮抗剂钙泊三醇C和PKCζ特异性抑制剂对胚胎早期转录激活的影响。在小鼠单细胞期受精卵中，PKC活性从G1期到G2晚期逐渐增加，并在G2期达到最大值。钙泊三醇C在单细胞期受精卵中抑制PKC活性约47%。钙泊三醇C抑制单细胞期受精卵的早期转录（p<0.01）。单细胞期受精卵G2期的PKCζ-Thr410分别比G1期和MII期卵母细胞高约27%和110%。PKCζ特异性抑制剂也能抑制单细胞期受精卵的早期转录（p<0.05）。结果表明，PKCζ参与小鼠单细胞期受精卵的早期转录激活。
