Epstein R J, Hendricks R L, Stulting R D
Department of Ophthalmology, Rush-Presbyterian-St. Luke's Medical Center, Atlanta, Georgia.
Cornea. 1990 Oct;9(4):318-23.
Mitogen-stimulated lymphocytes induce a highly reproducible form of corneal neovascularization (CNV) in inbred mice. To determine if supernatants derived from stimulated lymphocytes and their constituent mediators were also angiogenic, we injected conditioned medium (CM) from mitogen-stimulated lymphocytes, control non-conditioned medium, recombinant interleukin-2 (rIL-2), or control bovine serum albumin (BSA), a component of rIL-2, into the corneas of syngeneic A/J mice. Control, nonconditioned medium was not angiogenic. While the other injections all induced some CNV, the CM and rIL-2 both induced a significantly greater area of CNV than BSA (p less than 0.05). The area of CNV induced by the CM was greater than that induced by IL-2 (p = 0.03). These data show that IL-2, which stimulates vascular endothelial cells in vitro and is elaborated during corneal immune reactions in vivo, is one of the potential mediators of immunologically mediated CNV.
丝裂原刺激的淋巴细胞可在近交系小鼠中诱导出高度可重复的角膜新生血管形成（CNV）。为了确定来自刺激淋巴细胞的上清液及其组成介质是否也具有血管生成作用，我们将丝裂原刺激淋巴细胞的条件培养基（CM）、对照非条件培养基、重组白细胞介素-2（rIL-2）或对照牛血清白蛋白（BSA，rIL-2的一种成分）注射到同基因A/J小鼠的角膜中。对照非条件培养基不具有血管生成作用。虽然其他注射均诱导了一定程度的CNV，但CM和rIL-2诱导的CNV面积均显著大于BSA（p<0.05）。CM诱导的CNV面积大于IL-2诱导的面积（p = 0.03）。这些数据表明，IL-2在体外可刺激血管内皮细胞，且在体内角膜免疫反应过程中会大量产生，它是免疫介导的CNV的潜在介质之一。
