Escribá María-José, Martín Julio, Rubio Carmen, Valbuena Diana, Remohí José, Pellicer Antonio, Simón Carlos
IVI Foundation, IVI University, Valencia, Spain.
Fertil Steril. 2006 Dec;86(6):1601-7. doi: 10.1016/j.fertnstert.2006.04.047. Epub 2006 Oct 24.
To prove the efficiency of identification and removal of one of the surplus paternal pronuclei in dispermic IVF zygotes to obtain heteroparental blastocysts.
Experimental.
One hundred fourteen tripronucleated (3PN) embryos from conventional IVF.
PATIENT(S): After informed and signed consent, the patients from Instituto Valenciano Infertilidad (IVI), Valencia, donated their abnormally fertilized embryos.
INTERVENTION(S): Seventy-two embryos were diploidized by microsurgical removal of the pronucleus located at the farthest position to the second polar body. Forty-two 3PN embryos served as controls.
MAIN OUTCOME MEASURE(S): Survival and correction rate; in vitro development up to the blastocyst stage; X, Y, and 18 chromosome determination by triple fluorescent in situ hybridization and, inheritance analysis for 10 polymorphic repeat regions using polymerase chain reaction (PCR) amplification and sequencing.
RESULT(S): Seventy-eight percent of 3PN zygotes (56/72) survived manipulation and eventually 51 zygotes had two pronuclei (71%). Forty-one percent of manipulated embryos progressed in vitro to the blastocyst stage (21/51). Fluorescent in situ hybridization analysis performed on eight manipulated embryos confirmed their diploid state; all four controls were triploid. Heteroparental inheritances were also confirmed in four of six manipulated embryos.
CONCLUSION(S): Heteroparental blastocysts can be derived from corrected dispermic zygotes.
