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与核苷酸相关的杂环化合物。XI. 硫代尿苷的有机汞柱色谱法

Heterocycles related to nucleotides. XI. An organomercurial column chromatography for thiouridine.

作者信息

Sato E, Suzuki A, Kanaoka Y, Kimura K

机构信息

Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.

出版信息

J Pharmacobiodyn. 1990 Aug;13(8):487-92. doi: 10.1248/bpb1978.13.487.

DOI:10.1248/bpb1978.13.487
PMID:1706764
Abstract

By the organomercurial column: chromagel or Sephadex coupled to p-aminophenylmercury chloride, 4- and 2-thiouridine were selectively separated from the mixtures with major nucleotides. Oligonucleotides containing 4-thiouridilic acid were also separated from the ribonuclease-T1 digests of unfractionated transfer ribonucleic acid of E. coli.

摘要

通过有机汞柱

与对氨基苯基氯化汞偶联的色凝胶或葡聚糖凝胶，4-硫尿苷和2-硫尿苷能从含有主要核苷酸的混合物中被选择性分离出来。含有4-硫尿苷酸的寡核苷酸也能从不分级的大肠杆菌转移核糖核酸的核糖核酸酶-T1消化产物中被分离出来。

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