Schaumann Daniel H S, Tuischer Jens, Ebell Wolfram, Manz Rudolf A, Lauster Roland
Molecular Biology, German Arthritis Research Center, Berlin, Germany.
Mol Immunol. 2007 Mar;44(7):1606-12. doi: 10.1016/j.molimm.2006.08.021. Epub 2006 Oct 25.
The differentiation of B lymphocytes in the bone marrow is guided by the surrounding microenvironment determined by cytokines, adhesion molecules, and the extracellular matrix. These microenvironmental factors are mainly provided by stromal cells. In this paper, we report the identification of a VCAM-1-positive stromal cell population by flow cytometry. This population showed the expression of cell surface markers known to be present on stromal cells (CD10, CD13, CD90, CD105) and had a fibroblastoid phenotype in vitro. Single cell RT-PCR analysis of its cytokine expression pattern revealed transcripts for haematopoietic cytokines important for either the early B lymphopoiesis like flt3L or the survival of long-lived plasma cells like BAFF or both processes like SDF-1. Whereas SDF-1 transcripts were detectable in all VCAM-1-positive cells, flt3L and BAFF were only expressed by some cells suggesting the putative existence of different subpopulations with distinct functional properties. In summary, the VCAM-1-positive cell population seems to be a candidate stromal cell population supporting either developing B cells and/or long-lived plasma cells in human bone marrow.
骨髓中B淋巴细胞的分化受细胞因子、黏附分子和细胞外基质所决定的周围微环境的引导。这些微环境因子主要由基质细胞提供。在本文中，我们报告了通过流式细胞术鉴定出一种VCAM-1阳性基质细胞群体。该群体表现出已知存在于基质细胞上的细胞表面标志物（CD10、CD13、CD90、CD105）的表达，并且在体外具有成纤维细胞样表型。对其细胞因子表达模式进行单细胞RT-PCR分析，揭示了对早期B淋巴细胞生成（如flt3L）或长寿浆细胞存活（如BAFF）或两个过程（如SDF-1）都重要的造血细胞因子的转录本。虽然在所有VCAM-1阳性细胞中都可检测到SDF-1转录本，但flt3L和BAFF仅由一些细胞表达，这表明可能存在具有不同功能特性的不同亚群。总之，VCAM-1阳性细胞群体似乎是支持人类骨髓中正在发育的B细胞和/或长寿浆细胞的候选基质细胞群体。
