基质细胞衍生因子-1和CXCR4在子宫内膜癌组织及细胞系中的表达

[Expressions of stromal cell-derived factor-1 and CXCR4 in endometrial carcinoma tissues and cell lines].

作者信息

Li Xiao-ping, Zhao Dan, Gao Min, Wang Jian-liu, Wei Li-hui

机构信息

Department of Gynecology, Peking University Peopleos Hospital, Beijing 100044, China.

出版信息

Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Oct 18;38(5):458-62.

PMID:17068613

Abstract

OBJECTIVE

To investigate the expressions of SDF-1 and CXCR4 in human endometrial carcinoma tissues.

METHODS

SDF-1 and CXCR4 protein expression levels were detected by immunohistochemical staining in 26 cases of atypic hyperplasia endometrium,simple hyperplasia endometrium and normal endometrium and 44 cases of endometrial carcinoma tissues.

RESULTS

Both SDF-1 mRNA and CXCR4 mRNA were expressed in Ishikawa, while no expression of SDF-1 mRNA was detected in HEC-1A. The rates of CXCR4 and SDF-1 protein expressions were 100% in glandular cells of human normal endometrial tissues, while 90.9% and 93.2% in glandular cells of endometrial carcinoma tissues. These immunoreactivities of SDF-1 and CXCR4 were significantly low in endometrial carcinoma tissues as compared with those in normal endometrium; and the intensities of immunohistochemical staining significantly decreasesd in immunoreactivity of SDF-1 and CXCR4 in low grade as compared with that in hight grade.

CONCLUSION

SDF-1 and CXCR4 were expressed differently in endometrial cell lines, human normal endometrial tissues,and endometrial carcinoma tissues. As compared with normal endometrium ,these were significantly low levels expression of SDF-1 and CXCR4 in endometrial carcinoma tissues.

摘要

目的

探讨基质细胞衍生因子-1（SDF-1）和CXC趋化因子受体4（CXCR4）在人子宫内膜癌组织中的表达情况。

方法

采用免疫组织化学染色法检测26例不典型增生子宫内膜、单纯增生子宫内膜及正常子宫内膜组织和44例子宫内膜癌组织中SDF-1和CXCR4蛋白的表达水平。

结果

Ishikawa细胞中SDF-1 mRNA和CXCR4 mRNA均有表达，而HEC-1A细胞中未检测到SDF-1 mRNA的表达。人正常子宫内膜组织腺上皮细胞中CXCR4和SDF-1蛋白表达率均为100%，而子宫内膜癌组织腺上皮细胞中分别为90.9%和93.2%。与正常子宫内膜相比，子宫内膜癌组织中SDF-1和CXCR4的免疫反应性明显降低；与高分化子宫内膜癌相比，低分化子宫内膜癌组织中SDF-1和CXCR4免疫组化染色强度明显降低。