Yu Xiao-min, Liu Xin-min, Qi Yong-fen, Zhang Jing, Tang Chao-shu
Department of Geriatric Medicine, Peking University First Hospital, Beijing 100034, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Oct 18;38(5):496-500.
To investigate the changes of pulmonary IMD and its receptor system-calcitonin receptor-like receptor (CL) and receptor activity modifying proteins (RAMPs) mRNA in acute lung injury(ALI) induced by oleic acid of rats.
Contents of IMD in plasma and lung homogenates were measured by radioimmunoassay(RIA). The lung mRNA of IMD, CL and RAMPs was determined by semi-quantitative RT-PCR.
Compared with control group, in ALI group, the contents of IMD1-53 in plasma and lung homogenates were decreased by 20.8% and 74.5% (all P < 0.05) , respectively. Furthermore , it was found that the levels of IMD, CL, RAMP1 and RAMP2 mRNA in lung were decreased by 30%, 38%, 26% and 37.9% (all P < 0. 05) , respectively. The levels of CL , RAMP1 or RAMP2 mRNA were positively correlated with down-regulations of IMD mRNA in ALI. However, compared with control group, the maximum binding capacity of IMD1-53 to plasma membranes was significantly increased in ALI group, and the affinity of IMD1-53 for its receptor almost had no change.
The amount of IMD 1-53 is down-regulated and IMD receptor system also down-regulated in Oliec acid induced ALI of rats. These changes suggest that IMD and its receptor system probably are involved in the development of ALI.
研究油酸诱导大鼠急性肺损伤（ALI）时肺组织中免疫活性多肽（IMD）及其受体系统——降钙素受体样受体（CL）和受体活性修饰蛋白（RAMPs）mRNA的变化。
采用放射免疫分析法（RIA）检测血浆及肺匀浆中IMD含量。采用半定量逆转录聚合酶链反应（RT-PCR）检测肺组织中IMD、CL及RAMPs的mRNA。
与对照组相比，ALI组血浆及肺匀浆中IMD1-53含量分别降低20.8%和74.5%（均P<0.05）。此外，发现肺组织中IMD、CL、RAMP1及RAMP2的mRNA水平分别降低30%、38%、26%及37.9%（均P<0.05）。ALI时CL、RAMP1或RAMP2的mRNA水平与IMD mRNA的下调呈正相关。然而，与对照组相比，ALI组IMD1-53与质膜的最大结合容量显著增加，IMD1-53与其受体的亲和力几乎无变化。
油酸诱导大鼠ALI时，IMD 1-53含量下调，IMD受体系统也下调。这些变化提示IMD及其受体系统可能参与ALI的发生发展。
