Yu Ri-yue, Zhou Yong-sheng, Feng Hai-lan, Liu Xi-yun
Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Oct 18;38(5):522-4.
To evaluate the biocompatibility of polymethylmethacrylate(PMMA) denture base resin containing silver-supported antimicrobial agent STR-1 of nanometer level in vitro.
According to the national standards for biological evaluation of dental materials, the cytotoxicity of denture base resin containing STR-1 at concentrations of 5 g/L and 10 g/L was examined by molecular filtrating method, and the hemolysis of STR-1, denture base resin containing STR-1 at concentrations of 5 g/L and 10 g/L was also surveyed.
The control denture base resin without containing STR-1 and the denture base resins containing STR-1 at concentrations of 5 g/L and 10 g/L were not cytotoxic to L929 cells. Two hours and 24 hours after cell culturing, the filter membranes of the control and experimental groups were stained evenly with blue color. The staining intensity was not decreased and the fading areas were 0 mm2 during the culturing. The cytotoxicity grades were 0. The hemolysis rates of the antimicrobial agent STR-1 and the denture base resins containing STR-1 at concentrations of 5 g/L and 10 g/L were 1.7%, 3.5% and 3.7% respectively. They were less than the national guild standard 5% which represent no hemolysis.
The PMMA denture base resins containing silver-supported antimicrobial agents STR-1 of nanometer level at concentrations of 5 g/L and 10 g/L exhibit good biocompatibility.
体外评价含纳米级载银抗菌剂STR-1的聚甲基丙烯酸甲酯（PMMA）义齿基托树脂的生物相容性。
按照口腔材料生物学评价国家标准，采用分子滤过法检测含5 g/L和10 g/L STR-1的义齿基托树脂的细胞毒性，同时检测STR-1、含5 g/L和10 g/L STR-1的义齿基托树脂的溶血率。
不含STR-1的对照义齿基托树脂及含5 g/L和10 g/L STR-1的义齿基托树脂对L929细胞均无细胞毒性。细胞培养2小时及24小时后，对照组和实验组滤膜均被均匀染成蓝色，培养过程中染色强度未降低，褪色面积为0 mm²，细胞毒性分级为0级。抗菌剂STR-1及含5 g/L和10 g/L STR-1的义齿基托树脂的溶血率分别为1.7%、3.5%和3.7%，均小于国家行业标准5%，表明无溶血现象。
含5 g/L和10 g/L纳米级载银抗菌剂STR-1的PMMA义齿基托树脂具有良好的生物相容性。
