可溶性和颗粒性肌醇1,4,5-三磷酸5-磷酸酶显示出共同的抗原决定簇。

Soluble and particulate inositol 1,4,5-trisphosphate 5-phosphatases show common antigenic determinants.

作者信息

Verjans B, Hollande F, Moreau C, Lejeune C, Erneux C

机构信息

Institut de Recherche Interdisciplinaire (IRIBHN), Université Libre de Bruxelles, Brussels, Belgium.

出版信息

Cell Signal. 1990;2(6):595-9. doi: 10.1016/0898-6568(90)90082-l.

DOI:10.1016/0898-6568(90)90082-l

PMID:1706930

Abstract

Inositol 1,4,5-trisphosphate 5-phosphatase catalyses the dephosphorylation of the phosphate in the 5-position from inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate. One particulate and two soluble enzymes were previously described in bovine brain. In this study, we have obtained a precipitating antiserum against soluble type I inositol 1,4,5-trisphosphate 5-phosphatase. The particulate, but not the soluble type II enzyme, was immunoprecipitated by the serum. Inositol 1,4,5-trisphosphate 5-phosphatase activity from crude extracts of rat brain, human platelets and rat liver were immunoprecipitated by the same antibodies, suggesting the existence of common antigenic determinant among inositol 1,4,5-trisphosphate 5-phosphatases of diverse sources.

摘要

肌醇1,4,5-三磷酸5-磷酸酶催化从肌醇1,4,5-三磷酸和肌醇1,3,4,5-四磷酸中去除5位上的磷酸基团。先前在牛脑中描述了一种颗粒性酶和两种可溶性酶。在本研究中，我们获得了一种针对可溶性I型肌醇1,4,5-三磷酸5-磷酸酶的沉淀抗血清。该血清可免疫沉淀颗粒性酶，但不能沉淀可溶性II型酶。来自大鼠脑、人血小板和大鼠肝脏粗提物中的肌醇1,4,5-三磷酸5-磷酸酶活性也能被相同抗体免疫沉淀，这表明不同来源的肌醇1,4,5-三磷酸5-磷酸酶之间存在共同的抗原决定簇。