Zhang Gui-hong, Zeng Yu, Yang Wen-tao, Shi Da-ren
Department of Pathology, Cancer Hospital, Fu Dan University, Shanghai 200032, China.
Zhonghua Bing Li Xue Za Zhi. 2006 Aug;35(8):473-7.
To study mRNA expression of VEGF-(A, C, D) genes in breast carcinoma and its effect on prognosis.
The mRNA expression of VEGF-(A, C, D) genes in 61 cases of primary breast carcinoma and 29 cases of benign mammary lesions was detected by TaqMan real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) technology. The correlation between mRNA expression and various clinicopathologic parameters and survival data was analyzed.
The level of mRNA expression of VEGF-(A, C) genes was significantly higher in primary breast carcinomas (2.79 +/- 1.31 and 3.33 +/- 0.88 respectively) than in benign mammary lesions (1.59 +/- 1.35 and 2.76 +/- 0.55 respectively, P = 0.000 and 0.002 respectively). The percentage of VEGF-D mRNA expression was 73.77% in breast carcinomas, as compared to 51.72% in benign mammary lesions (P = 0.038). However, there was no statistically significant difference in the mRNA expression levels of VEGF-D gene between primary breast carcinomas and benign mammary lesions (P = 0.683). On the other hand, the ratio of VEGF-D mRNA expression to VEGF-C mRNA expression was lower in lymph node-positive than in lymph node-negative cases. This also correlated with the occurrence of nodal metastasis by uni- and multivariate analysis (P(uni) = 0.046 and P(mult) = 0.062). High mRNA expression of VEGF-(A, C) genes was associated with poor disease-free survival (P = 0.030 and 0.044 respectively).
The expression of VEGF-(A, C, D) genes may play a role in the disease progression of breast carcinoma. The ratio of VEGF-D mRNA expression to VEGF-C mRNA expression correlates with the occurrence of lymph node metastasis in breast carcinoma. The mRNA expression of VEGF-(A, C) genes may serve as a useful prognostic indicator in breast cancer.
研究VEGF-（A、C、D）基因在乳腺癌中的mRNA表达及其对预后的影响。
采用TaqMan实时逆转录-聚合酶链反应（实时RT-PCR）技术检测61例原发性乳腺癌和29例乳腺良性病变中VEGF-（A、C、D）基因的mRNA表达。分析mRNA表达与各种临床病理参数及生存数据之间的相关性。
原发性乳腺癌中VEGF-（A、C）基因的mRNA表达水平（分别为2.79±1.31和3.33±0.88）显著高于乳腺良性病变（分别为1.59±1.35和2.76±0.55，P分别为0.000和0.002）。乳腺癌中VEGF-D mRNA表达的百分比为73.77%，而乳腺良性病变中为51.72%（P = 0.038）。然而，原发性乳腺癌和乳腺良性病变之间VEGF-D基因的mRNA表达水平无统计学显著差异（P = 0.683）。另一方面，淋巴结阳性病例中VEGF-D mRNA表达与VEGF-C mRNA表达的比值低于淋巴结阴性病例。单因素和多因素分析也表明这与淋巴结转移的发生相关（单因素P = 0.046，多因素P = 0.062）。VEGF-（A、C）基因的高mRNA表达与无病生存期差相关（分别为P = 0.030和0.044）。
VEGF-（A、C、D）基因的表达可能在乳腺癌的疾病进展中起作用。VEGF-D mRNA表达与VEGF-C mRNA表达的比值与乳腺癌淋巴结转移的发生相关。VEGF-（A、C）基因的mRNA表达可能作为乳腺癌有用的预后指标。
