Gonçalves Margareth M L, Barreto Magali G M, Peralta Regina H S, Gargioni Cybele, Gonçalves Tiana, Igreja Ricardo P, Soares Marisa S, Peralta José M
Departamento de Imunologia, Instituto de Microbiologia Professor Paulo de Góes-CCS, Universidade Federal do Rio de Janeiro, 21941590 Rio de Janeiro, Brazil.
Acta Trop. 2006 Nov;100(1-2):24-30. doi: 10.1016/j.actatropica.2006.09.004. Epub 2006 Oct 27.
In an attempt to improve the screening of Schistosoma mansoni-infected individuals from a low-transmission area, we established a protocol that includes three independent coproscopy examinations and two serological assays. Three stool samples were examined using the Kato-Katz and free sedimentation methods and serum samples were tested by IgG-ELISA and IgM-immunofluorescence. Two hundred and sixty-nine individuals participated in the survey: 132 individuals (49%) showed positive serological test results. Of these, 16 (6%) had positive results in stool examination in the first sample batch. However, there were also cases with positive serological test results in spite of negative Kato-Katz stool examinations. Additional stool samples were obtained from these subjects and in this way an additional 11 egg-excretors were found. Our findings suggest that a screening method that combines antibody isotype detection and repeated parasitological stool examinations could increase the chances of detecting S. mansoni-infected patients.
为了改进对来自低传播地区曼氏血吸虫感染个体的筛查，我们制定了一个方案，该方案包括三项独立的粪便检查和两项血清学检测。使用加藤厚涂片法和自由沉淀法检查三份粪便样本，并用IgG-ELISA和IgM免疫荧光法检测血清样本。269人参与了此次调查：132人（49%）血清学检测结果呈阳性。其中，16人（6%）在第一批样本的粪便检查中结果呈阳性。然而，也有一些病例尽管加藤厚涂片粪便检查结果为阴性，但血清学检测结果呈阳性。从这些受试者那里获取了额外的粪便样本，通过这种方式又发现了11名排虫卵者。我们的研究结果表明，一种结合抗体同种型检测和重复寄生虫学粪便检查的筛查方法可以增加检测出曼氏血吸虫感染患者的几率。
