Cheikh Amani, Benkhalifa Rym, Potreau Daniel, Raymond Guy, El Ayeb Mohamed, Cognard Christian
Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, Tunis BP 74-1002, Tunisia.
Toxicon. 2007 Jan;49(1):117-22. doi: 10.1016/j.toxicon.2006.09.011. Epub 2006 Sep 22.
A depolarizing activity following interaction with nicotinic acetylcholine receptors (nAchRs) in skeletal muscle cells, was observed for the first time in the non-toxic venom fraction (M1) of the yellow scorpion Buthus occitanus tunetanus (Bot). The effects of M1 fraction were tested on cultured rat myotubes by recording changes in [Ca2+]i. When applied, M1 (10 microg/mL) induced a transient increase of [Ca2+]i which could be blocked by a prior application of alpha-Bungarotoxin (alpha-Bg-Tx).
首次在黄色蝎子突尼斯金蝎（Bot）的无毒毒液组分（M1）中观察到，与骨骼肌细胞中的烟碱型乙酰胆碱受体（nAchRs）相互作用后出现去极化活性。通过记录细胞内钙离子浓度（[Ca2+]i）的变化，检测了M1组分对培养的大鼠肌管的影响。施加M1（10微克/毫升）时，可诱导[Ca2+]i短暂升高，预先施加α-银环蛇毒素（α-Bg-Tx）可阻断这种升高。
