Osanto S, Thijssen J C, Woldering V M, van Rijn J L, Natarajan A T, Tates A D
Department of Clinical Oncology, University Hospital Leiden, The Netherlands.
Environ Mol Mutagen. 1991;17(2):71-8. doi: 10.1002/em.2850170202.
The presence of micronuclei (MN) in binucleated peripheral blood lymphocytes of 37 testicular carcinoma patients was studied 1.5 to 9.3 years following curative chemotherapy. All patients received cisplatinum, 35 patients also received bleomycin. In addition, most patients were treated with another cytotoxic drug, i.e., vinblastine (n = 23) and/or etoposide (n = 24). The mean time interval between cessation of chemotherapy and the micronucleus assay was 4.6 years. The median frequency of MN in binucleated cells in treated patients (0.059) was significantly higher than that in 12 untreated cancer patients (0.036; P = 0.003) or that in 26 healthy age-matched controls (0.034; P less than 0.001). Frequencies of MN in the 12 untreated cancer patients (including 7 patients with disseminated testicular carcinoma) did not differ from those in the 26 healthy controls (P = 0.890), suggesting that chromosomal damage in lymphocytes of treated testicular cancer patients must be attributed to the chemotherapy. Results indicate that lymphocytes containing chemically induced chromosomal damage persist for up to 9.3 years following cessation of chemotherapy. The implications of these findings with regard to the increased risk for secondary tumors are discussed.
对37例睾丸癌患者在根治性化疗后1.5至9.3年的双核外周血淋巴细胞中的微核（MN）情况进行了研究。所有患者均接受了顺铂治疗，35例患者还接受了博来霉素治疗。此外，大多数患者还接受了另一种细胞毒性药物治疗，即长春碱（n = 23）和/或依托泊苷（n = 24）。化疗结束至微核检测的平均时间间隔为4.6年。治疗患者双核细胞中MN的中位频率（0.059）显著高于12例未治疗的癌症患者（0.036；P = 0.003）或26例年龄匹配的健康对照者（0.034；P < 0.001）。12例未治疗的癌症患者（包括7例播散性睾丸癌患者）的MN频率与26例健康对照者的频率无差异（P = 0.890），这表明接受治疗的睾丸癌患者淋巴细胞中的染色体损伤一定归因于化疗。结果表明，含有化学诱导染色体损伤的淋巴细胞在化疗停止后可持续长达9.3年。讨论了这些发现对于继发性肿瘤风险增加的意义。
