Zhang Ai-Hua, Chen Min, Gao Ying, Zhao Ming-Hui, Wang Hai-Yan
Renal Division and Institute of Nephrology, Peking University First Hospital, Beijing 100034, PR China.
Clin Immunol. 2007 Feb;122(2):187-93. doi: 10.1016/j.clim.2006.09.011. Epub 2006 Oct 27.
Propylthiouracil (PTU) could induce antineutrophil cytoplasmic antibody (ANCA) associated vasculitis. This study aimed to investigate the inhibitory effects on MPO oxidation activity by PTU and MPO-ANCA from patients with primary microscopic polyangiitis (MPA) and PTU-induced vasculitis. IgG preparations were purified from MPO-ANCA-positive sera from seven patients with PTU-induced vasculitis and ten patients with primary MPA. The oxidation activity of MPO was measured in the presence of PTU and MPO-ANCA-positive IgG preparations from patients with PTU-induced vasculitis and primary MPA respectively. PTU could competitively inhibit the oxidation activity of MPO dose dependently. MPO-ANCA-positive IgG preparations from 6/7 patients with PTU-induced vasculitis and only 3/10 from patients with primary MPA could inhibit the MPO activity in a dose-dependent manner. In conclusions, the oxidation activity of MPO could be inhibited by PTU and PTU-induced MPO-ANCA in a dose-dependent manner, which might be involved in the pathogenesis PTU-induced vasculitis.
丙硫氧嘧啶（PTU）可诱发抗中性粒细胞胞浆抗体（ANCA）相关血管炎。本研究旨在探讨PTU以及原发性显微镜下多血管炎（MPA）患者和PTU诱导的血管炎患者的MPO-ANCA对MPO氧化活性的抑制作用。从7例PTU诱导的血管炎患者和10例原发性MPA患者的MPO-ANCA阳性血清中纯化IgG制剂。分别在PTU以及PTU诱导的血管炎患者和原发性MPA患者的MPO-ANCA阳性IgG制剂存在的情况下测量MPO的氧化活性。PTU可剂量依赖性地竞争性抑制MPO的氧化活性。7例PTU诱导的血管炎患者中有6例的MPO-ANCA阳性IgG制剂以及原发性MPA患者中只有3/10的制剂可剂量依赖性地抑制MPO活性。总之，PTU和PTU诱导的MPO-ANCA可剂量依赖性地抑制MPO的氧化活性，这可能与PTU诱导的血管炎的发病机制有关。
