Robl J M, Wang Z, Kasinathan P, Kuroiwa Y
Hematech Inc, Sioux Falls, SD 57106, USA.
Theriogenology. 2007 Jan 1;67(1):127-33. doi: 10.1016/j.theriogenology.2006.09.034. Epub 2006 Oct 27.
Considerable progress has been made in methods for production of transgenic livestock; beginning with pronuclear microinjection over 20 years ago. New methods, including the use of viral vectors, sperm-mediated gene transfer and somatic cell cloning, have overcome many of the limitations of pronuclear microinjection. It is now possible to not only readily make simple insertional genetic modifications, but also to accomplish, more complex, homozygous gene targeting and artificial chromosome transfer in livestock.
在转基因家畜的生产方法方面已经取得了相当大的进展；这始于20多年前的原核显微注射。新方法，包括使用病毒载体、精子介导的基因转移和体细胞克隆，已经克服了原核显微注射的许多局限性。现在不仅可以轻松地进行简单的插入性基因改造，还可以在牲畜中完成更复杂的纯合基因靶向和人工染色体转移。
