Hayes J R, Meckley D R, Stavanja M S, Nelson P R, Van Kampen K R, Swauger J E
RJ Reynolds Tobacco Company, Bowman Gray Technical Center, Research and Development, Scientific and Regulatory Affairs, Winston-Salem, NC 27102-1487, USA.
Food Chem Toxicol. 2007 Mar;45(3):419-30. doi: 10.1016/j.fct.2006.08.024. Epub 2006 Sep 14.
A 30-week dermal tumor promotion study was conducted to evaluate the dermal tumor-promoting potential of cigarette smoke condensate (CSC) collected from cigarettes containing flue-cured tobacco cured by a heat-exchange process (HE) relative to that of cigarettes containing flue-cured tobacco cured by the traditional direct-fire process (DF). Heat-exchange process cured tobacco contains significantly lower concentrations of tobacco specific nitrosamines (TSNAs) compared to traditional direct-fire cured tobacco. Mainstream CSCs were collected by cold trap from smoke generators using the Federal Trade Commission puffing regimen. Groups of 40 female SENCAR mice were initiated by a single application of 75 micro g 7,12-dimethylbenz[a]anthracene (DMBA) to the shaved dorsal skin. CSCs were then applied to the skin three times/week for 29 weeks at 9, 18, or 36mg tar/application. End-points included body weights, clinical observations, organ weights, dermal tumor development and histopathology. The numbers of dermal tumors and the numbers of tumor-bearing mice for each CSC were statistically different from the DMBA/acetone control group and increased with increasing dose. When corresponding doses of each CSC were compared, only the DMBA/mid-dose HE CSC group was statistically significantly different (lower) from the corresponding DMBA/mid-dose DF CSC group. In this assay, the dermal tumor-promotion potential of CSC from heat-exchange flue-cured tobacco did not differ from that of traditional direct-fire flue-cured tobacco CSC.
进行了一项为期30周的皮肤肿瘤促进研究，以评估从采用热交换工艺（HE）烤制的烤烟制成的香烟中收集的香烟烟雾冷凝物（CSC）相对于采用传统直火工艺（DF）烤制的烤烟制成的香烟的CSC的皮肤肿瘤促进潜力。与传统直火烤制的烟草相比，热交换工艺烤制的烟草中烟草特异性亚硝胺（TSNA）的浓度显著更低。使用联邦贸易委员会抽吸方案，通过冷阱从烟雾发生器中收集主流CSC。40只雌性SENCAR小鼠组成的各组通过在剃毛的背部皮肤单次涂抹75微克7,12-二甲基苯并[a]蒽（DMBA）启动。然后，CSC以9、18或36毫克焦油/次的剂量每周三次涂抹于皮肤上，持续29周。终点指标包括体重、临床观察、器官重量、皮肤肿瘤发展和组织病理学。每种CSC的皮肤肿瘤数量和荷瘤小鼠数量与DMBA/丙酮对照组在统计学上存在差异，且随剂量增加而增加。当比较每种CSC的相应剂量时，只有DMBA/中剂量HE CSC组与相应的DMBA/中剂量DF CSC组在统计学上有显著差异（更低）。在该试验中，热交换烤制烟草的CSC的皮肤肿瘤促进潜力与传统直火烤制烟草的CSC没有差异。
