Tuncel Deniz, Aydin Nursel, Aribal Kocatürk Pelin, Ozelçi Kavas Güzin, Sarikaya Sevda
Department of Neurology, Faculty of Medicine, Kahramanmaras University, Kahramanmaras, Turkey.
J Clin Neurosci. 2006 Dec;13(10):991-4. doi: 10.1016/j.jocn.2005.10.019. Epub 2006 Oct 30.
Specific biologic markers are not available for definitive diagnosis and monitoring of disease progression in sporadic amyotrophic lateral sclerosis (SALS). Oxidative stress plays a role in ALS pathogenesis. The purpose of this study was to determine the association between Cu/Zn superoxide dismutase (SOD1) activity, diagnosis and prognosis. The present study included 25 SALS patients (SALS group; age 51+/-12 years) and 10 healthy subjects (age 45+/-5 years) as a control group. Patients were divided into groups representing four levels of diagnostic certainty of ALS in accordance with the El Escorial Revisited criteria. The disease state was determined using the modified ALS health state scale of Riviere et al. (Arch Neurol 1998:55;526-8). Red-cell SOD1 activity was determined by spectrophotometry. SOD1 activity in red cells was compared statistically with diagnostic criteria and disease state. Red cell SOD1 activity was high in all SALS patients, but there was no significant association between enzyme activity and diagnostic criteria and disease state. In this preliminary study, we did not find any correlation between SOD1 activity level and diagnosis or prognosis. Measured SOD1 activity sometimes supports ALS diagnosis, but it is neither a specific nor a prognostic factor.
在散发性肌萎缩侧索硬化症（SALS）中，尚无用于明确诊断和监测疾病进展的特异性生物标志物。氧化应激在肌萎缩侧索硬化症的发病机制中起作用。本研究的目的是确定铜/锌超氧化物歧化酶（SOD1）活性与诊断及预后之间的关联。本研究纳入了25例SALS患者（SALS组；年龄51±12岁）和10名健康受试者（年龄45±5岁）作为对照组。根据修订的埃斯科里亚尔标准，将患者分为代表肌萎缩侧索硬化症诊断确定性四个水平的组。使用Riviere等人修改后的肌萎缩侧索硬化症健康状态量表（《神经病学档案》1998年；55卷；526 - 528页）确定疾病状态。通过分光光度法测定红细胞SOD1活性。将红细胞中的SOD1活性与诊断标准和疾病状态进行统计学比较。所有SALS患者的红细胞SOD1活性均较高，但酶活性与诊断标准和疾病状态之间无显著关联。在这项初步研究中，我们未发现SOD1活性水平与诊断或预后之间存在任何相关性。所测得的SOD1活性有时支持肌萎缩侧索硬化症的诊断，但它既不是特异性因素也不是预后因素。
