FtsN非必需N端在分裂体组装中的作用。
Role for the nonessential N terminus of FtsN in divisome assembly.
作者信息
Goehring Nathan W, Robichon Carine, Beckwith Jon
机构信息
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.
出版信息
J Bacteriol. 2007 Jan;189(2):646-9. doi: 10.1128/JB.00992-06. Epub 2006 Oct 27.
Abstract
FtsN, the last essential protein in the cell division localization hierarchy in Escherichia coli, has several peculiar characteristics, suggesting that it has a unique role in the division process despite the fact that it is conserved in only a subset of bacteria. In addition to suppressing temperature-sensitive mutations in ftsA, ftsK, ftsQ, and ftsI, overexpression of FtsN can compensate for a complete lack of FtsK in the cell. We examined the requirements for this phenomenon. We found that the N-terminal terminal region (cytoplasmic and transmembrane domains) is critical for suppression, while the C-terminal murein-binding domain is dispensable. Our results further suggest that FtsN and FtsK act cooperatively to stabilize the divisome.
摘要
FtsN是大肠杆菌细胞分裂定位层级中最后一个必需蛋白，它具有几个独特的特征，这表明尽管它仅在一部分细菌中保守，但在分裂过程中具有独特作用。除了抑制ftsA、ftsK、ftsQ和ftsI中的温度敏感突变外，FtsN的过表达可以弥补细胞中完全缺乏FtsK的情况。我们研究了这种现象的条件。我们发现N端区域（细胞质和跨膜结构域）对于抑制至关重要，而C端胞壁质结合结构域则是可有可无的。我们的结果进一步表明，FtsN和FtsK协同作用以稳定分裂体。
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本文引用的文献
1
Mutants, suppressors, and wrinkled colonies: mutant alleles of the cell division gene ftsQ point to functional domains in FtsQ and a role for domain 1C of FtsA in divisome assembly.突变体、抑制子与皱缩菌落：细胞分裂基因ftsQ的突变等位基因指向FtsQ中的功能结构域以及FtsA的1C结构域在分裂体组装中的作用。
J Bacteriol. 2007 Jan;189(2):633-45. doi: 10.1128/JB.00991-06. Epub 2006 Sep 15.
2
Premature targeting of cell division proteins to midcell reveals hierarchies of protein interactions involved in divisome assembly.细胞分裂蛋白过早靶向细胞中部揭示了参与分裂体组装的蛋白质相互作用层次结构。
Mol Microbiol. 2006 Jul;61(1):33-45. doi: 10.1111/j.1365-2958.2006.05206.x.
3
Evidence for functional overlap among multiple bacterial cell division proteins: compensating for the loss of FtsK.多种细菌细胞分裂蛋白功能重叠的证据：弥补FtsK的缺失。
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4
Interaction network among Escherichia coli membrane proteins involved in cell division as revealed by bacterial two-hybrid analysis.通过细菌双杂交分析揭示的参与细胞分裂的大肠杆菌膜蛋白之间的相互作用网络。
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Use of a two-hybrid assay to study the assembly of a complex multicomponent protein machinery: bacterial septosome differentiation.利用双杂交试验研究复杂多组分蛋白质机器的组装：细菌隔膜体分化
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