Fernandez C, Möller G
Department of Immunology, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden.
Scand J Immunol. 1991 Mar;33(3):307-17. doi: 10.1111/j.1365-3083.1991.tb01776.x.
A collection of hybridomas from C57BL/6 mice producing antibodies to dextran B512 was analysed and found to reflect the immune response in vivo with regard to immunoglobulin class expression, T cell dependency and antibody affinity. IgM-, IgG-3, and IgG-2b, and IgA-producing hybridomas were found. IgG3-producing hybridomas were obtained from nude mice, indicating T cell independent IgG3 synthesis. All monoclonal antibodies were of kappa light chain. A major anti-dextran idiotype was expressed in many monoclonals. Secondary immune responses to dextran were also suppressed at the hybridoma cell level. However, hybridomas from secondary responses produced antibodies expressing the major idiotype, suggesting that anti-idiotype mediated suppression was not responsible for the reduced secondary response. Most monoclonals belonged to the VHJ558 family, but the IgG3-producing hybridomas showed a preferential use of genes from the VHX24 family. All monoclonals were directed against internal structures of the dextran molecule. The affinity for dextran of the IgG antibodies produced in secondary immune responses was drastically increased, even when the mice were immunized with thymus-independent forms of dextran, indicating that T helper cells need not be involved in affinity maturation of the immune response.
对来自C57BL/6小鼠、产生抗右旋糖酐B512抗体的杂交瘤进行了分析，发现其在免疫球蛋白类别表达、T细胞依赖性和抗体亲和力方面反映了体内的免疫反应。发现了产生IgM、IgG-3、IgG-2b和IgA的杂交瘤。从裸鼠中获得了产生IgG3的杂交瘤，表明IgG3的合成不依赖T细胞。所有单克隆抗体均为κ轻链。许多单克隆抗体表达了一种主要的抗右旋糖酐独特型。在杂交瘤细胞水平上，对右旋糖酐的二次免疫反应也受到抑制。然而，二次反应产生的杂交瘤产生了表达主要独特型的抗体，这表明抗独特型介导的抑制并不是二次反应降低的原因。大多数单克隆抗体属于VHJ558家族，但产生IgG3的杂交瘤优先使用VHx24家族的基因。所有单克隆抗体均针对右旋糖酐分子的内部结构。即使在用非胸腺依赖性形式的右旋糖酐免疫小鼠时，二次免疫反应产生的IgG抗体对右旋糖酐的亲和力也会大幅增加，这表明T辅助细胞无需参与免疫反应的亲和力成熟。
