通过转化相关重组克隆对哺乳动物基因组进行结构和功能分析，从而选择性分离大的染色体区域。

Selective isolation of large chromosomal regions by transformation-associated recombination cloning for structural and functional analysis of mammalian genomes.

作者信息

Kouprina Natalay, Noskov Vladimir N, Larionov Vladimir

机构信息

Laboratory of Biosystems and Cancer, National Cancer Institute, NIH, Bethesda, MD, USA.

出版信息

Methods Mol Biol. 2006;349:85-101. doi: 10.1385/1-59745-158-4:85.

DOI:10.1385/1-59745-158-4:85

PMID:17071976

Abstract

Transformation-associated recombination (TAR) cloning allows selective isolation of full-size genes and genomic loci as circular yeast artificial chromosomes in yeast. The method has a broad application for structural and functional genomics, long-range haplotyping, characterization of chromosomal rearrangements, and evolutionary studies. This chapter describes a basic protocol of gene isolation by TAR, as well as a method of conversion of TAR isolates into bacterial artificial chromosomes.

摘要

转化相关重组（TAR）克隆可在酵母中作为环状酵母人工染色体选择性分离全长基因和基因组位点。该方法在结构和功能基因组学、长程单倍型分析、染色体重排表征及进化研究等方面有广泛应用。本章介绍了通过TAR进行基因分离的基本方案，以及将TAR分离物转化为细菌人工染色体的方法。