Kouprina Natalay, Larionov Vladimir
Laboratory of Biosystems and Cancer, National Cancer Institute, National Institute of Health, Building 37, Room 5032, 9000 Rockville Pike, Bethesda, Maryland 20892, USA.
Nat Rev Genet. 2006 Oct;7(10):805-12. doi: 10.1038/nrg1943.
The structural and functional analysis of mammalian genomes would benefit from the ability to isolate from multiple DNA samples any targeted chromosomal segment that is the size of an average human gene. A cloning technique that is based on transformation-associated recombination (TAR) in the yeast Saccharomyces cerevisiae satisfies this need. It is a unique tool to selectively recover chromosome segments that are up to 250 kb in length from complex genomes. In addition, TAR cloning can be used to characterize gene function and genome variation, including polymorphic structural rearrangements, mutations and the evolution of gene families, and for long-range haplotyping.
对哺乳动物基因组进行结构和功能分析，若能从多个DNA样本中分离出任何与平均人类基因大小相当的目标染色体片段，将大有裨益。基于酿酒酵母中转化相关重组（TAR）的克隆技术满足了这一需求。它是一种独特的工具，可从复杂基因组中选择性回收长度达250 kb的染色体片段。此外，TAR克隆可用于表征基因功能和基因组变异，包括多态性结构重排、突变和基因家族的进化，还可用于长程单倍型分型。
