Katayama Takeshi, Yoshiyama Shinji, Tanaka Hideyuki, Wang Hong Hui, Nakamura Akio, Kohama Kazuhiro
Department of Molecular and Cellular Pharmacology, Gunma University Graduate School of Medicine, Gunma, Japan.
J Pharmacol Sci. 2006 Nov;102(3):339-42. doi: 10.1254/jphs.sc0060104. Epub 2006 Oct 28.
We prepared a cell-populated collagen-gel fiber including GbaSM-4 cells derived from the basilar artery of guinea pigs. This fiber tended to be a differentiated contractile phenotype in electron-microscope observations. Sphingosylphosphorylcholine (SPC) can induce contraction of the fiber (EC50 = 0.70 +/- 0.05 microM), and blebbistatin can inhibit the SPC-induced contraction (IC50 = 22.8 +/- 1.26 microM). Phosphorylation of the 20 kD myosin light chain (MLC20) significantly increased in GbaSM-4 cells provided with 1 microM SPC (P<0.05), which was maintained in the presence of 1 to 100 microM blebbistatin. These results suggest that vascular smooth muscle can relax even if MLC20 is phosphorylated.
我们制备了一种包含源自豚鼠基底动脉的GbaSM - 4细胞的细胞填充胶原凝胶纤维。在电子显微镜观察中，这种纤维倾向于呈现分化的收缩表型。鞘氨醇磷酸胆碱（SPC）可诱导该纤维收缩（半数有效浓度EC50 = 0.70 +/- 0.05微摩尔），而肌球蛋白磷酸酶抑制剂（blebbistatin）可抑制SPC诱导的收缩（半数抑制浓度IC50 = 22.8 +/- 1.26微摩尔）。在给予1微摩尔SPC的GbaSM - 4细胞中，20 kD肌球蛋白轻链（MLC20）的磷酸化显著增加（P<0.05），在存在1至100微摩尔肌球蛋白磷酸酶抑制剂的情况下这种增加得以维持。这些结果表明，即使MLC20发生磷酸化，血管平滑肌仍可松弛。
