Koperdáková Jana, Kosuth Ján, Cellárová Eva
Faculty of Science, Institute of Biology and Ecology, P. J. Safárik University, Mánesova, Kosice, Slovakia.
J Plant Res. 2007 Jan;120(1):123-8. doi: 10.1007/s10265-006-0031-6. Epub 2006 Oct 28.
The content of hypericins in in vitro regenerated Hypericum perforatum plants (R (0)) and four generations of their seed progeny (R (1)-R (4)) was compared. The mean content of hypericins in field-grown plants over the period 1992-2002 gradually increased under selection, and in the R (4) generation it was almost seven-times higher than that in the R (0) somaclones. Significant difference between hypericin content in diploids and tetraploids was detected in R (0), R (1) and R (3) generations. Hypericin content in four diploid and tetraploid lineages originated from a single somaclone was genotype dependent. To eliminate the influence of environmental conditions during different growing seasons, we used seeds of selected R (0)-R (3) plants to derive R'(1) to R'(4) generations cultivated during the same years. In this case no statistically significant difference in hypericin content was found between the R'(1)-R'(4) generations. Apomictically and sexually derived plants were distinguished by PCR using variable numbers of tandem repeats (VNTR) primers. The content of hypericins in apomictically derived progenies was compared.
比较了体外再生的贯叶连翘植株（R(0)）及其四代种子后代（R(1)-R(4)）中金丝桃素的含量。在1992年至2002年期间，经过选择，田间种植植株中金丝桃素的平均含量逐渐增加，在R(4)代中，其含量几乎是R(0)体细胞克隆植株的七倍。在R(0)、R(1)和R(3)代中，检测到二倍体和四倍体中金丝桃素含量存在显著差异。源自单个体细胞克隆的四个二倍体和四倍体系谱中的金丝桃素含量取决于基因型。为了消除不同生长季节环境条件的影响，我们使用选定的R(0)-R(3)植株的种子培育出在同一年种植的R'(1)至R'(4)代。在这种情况下，R'(1)-R'(4)代之间未发现金丝桃素含量有统计学上的显著差异。使用可变数量串联重复序列（VNTR）引物通过PCR区分无融合生殖和有性繁殖产生的植株。比较了无融合生殖产生的后代中金丝桃素的含量。
