Sergeyev D S, Godovikova T S, Zarytova V F
Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk.
FEBS Lett. 1991 Mar 25;280(2):271-3. doi: 10.1016/0014-5793(91)80309-q.
Bleomycin A5 oligonucleotide derivative was used for direct cleavage of a DNA target. In the presence of Fe2+ ions and 2-mercaptoethanol, Blm-R-pd(CCAAACA) (I) damaged the target. pd(TGTTTGGCGAAGGA), with the yield of 80% , without affecting its own oligonucleotide tail. The sites of cleavage were T3-T5 and G6-G7. Unbound bleomycin A5 damaged the G6-G7-C8 site. Reagent I formed more stable complementary complexes with the target than parent oligonucleotide (ATm = 11 C).
博来霉素A5寡核苷酸衍生物用于直接切割DNA靶标。在Fe2+离子和2-巯基乙醇存在的情况下，Blm-R-pd(CCAAACA)（I）会损伤靶标。pd(TGTTTGGCGAAGGA)，产率为80%，且不影响其自身的寡核苷酸尾部。切割位点为T3-T5和G6-G7。未结合的博来霉素A5会损伤G6-G7-C8位点。试剂I与靶标形成的互补复合物比亲本寡核苷酸更稳定（熔解温度为11℃）。
