Wolff Jeremy J, Amster I Jonathan, Chi Lianli, Linhardt Robert J
Department of Chemistry, University of Georgia, Athens, Georgia 30602, USA.
J Am Soc Mass Spectrom. 2007 Feb;18(2):234-44. doi: 10.1016/j.jasms.2006.09.020. Epub 2006 Oct 30.
The first application of electron detachment dissociation (EDD) to carbohydrates is presented. The structural characterization of glycosaminoglycan (GAG) oligosaccharides by mass spectrometry is a longstanding problem because of the lability of these acidic, polysulfated carbohydrates. Doubly-charged negative ions of four GAG tetrasaccharides are examined by EDD, collisionally activated dissociation (CAD), and infrared multiphoton dissociation (IRMPD). EDD is found to produce information-rich mass spectra with both cross ring and glycosidic cleavage product ions. In contrast, most of the product ions produced by CAD and IRMPD result from glycosidic cleavage. EDD shows great potential as a tool for locating the sites of sulfation and other modifications in glycosaminoglycan oligosaccharides.
本文展示了电子脱附解离(EDD)在碳水化合物上的首次应用。由于这些酸性多硫酸化碳水化合物的不稳定性,通过质谱对糖胺聚糖(GAG)寡糖进行结构表征一直是个长期存在的问题。利用EDD、碰撞诱导解离(CAD)和红外多光子解离(IRMPD)对四种GAG四糖的双电荷负离子进行了研究。结果发现,EDD能产生包含交叉环裂解和糖苷键裂解产物离子的信息丰富的质谱图。相比之下,CAD和IRMPD产生的大多数产物离子是由糖苷键裂解产生的。EDD作为一种定位糖胺聚糖寡糖中硫酸化位点和其他修饰位点的工具,具有巨大潜力。