Stewart K N, Roy-Chaudhury P, Lumsden L, Jones M C, Brown P A, Macleod A M, Haites N E, Simpson J G, Power D A
Department of Medicine and Therapeutics, University of Aberdeen, U.K.
J Pathol. 1991 Mar;163(3):265-72. doi: 10.1002/path.1711630313.
The aim of this study was to produce monoclonal antibodies to cultured human glomerular mesangial cells in order to obtain specific markers for these cells and to aid the study of their function. Using standard monoclonal antibody techniques, 29 hybridomas producing antibodies directed to cultured mesangial cells were obtained. Most of these antibodies were not reactive with normal or neoplastic haematopoietic cell lines by flow cytometry. Fourteen of the 29 culture supernatants bound to various components of normal human kidney sections stained by the alkaline phosphatase/anti-alkaline phosphatase (APAAP) method. Ten of these supernatants reacted with components within the glomerulus, with six binding to the mesangium. These studies suggest that (1) mesangial cells in culture may show significant de-differentiation, because most supernatants which reacted with mesangial cells in culture did not do so in tissue sections; (2) antibodies reactive with haematopoietic cells may not detect the majority of immunogenic surface antigens on cells in tissues; and (3) some of the antibodies which we have produced may prove to be useful markers for mesangial cells in glomerular disease.
本研究的目的是制备针对培养的人肾小球系膜细胞的单克隆抗体，以获得这些细胞的特异性标志物，并有助于对其功能的研究。采用标准的单克隆抗体技术，获得了29株产生针对培养系膜细胞抗体的杂交瘤。通过流式细胞术，这些抗体中的大多数与正常或肿瘤性造血细胞系无反应。29种培养上清液中的14种与用碱性磷酸酶/抗碱性磷酸酶（APAAP）方法染色的正常人肾切片的各种成分结合。其中10种上清液与肾小球内的成分反应，6种与系膜结合。这些研究表明：（1）培养的系膜细胞可能显示出显著的去分化，因为大多数与培养的系膜细胞反应的上清液在组织切片中未出现这种情况；（2）与造血细胞反应的抗体可能无法检测到组织中细胞上的大多数免疫原性表面抗原；（3）我们产生的一些抗体可能被证明是肾小球疾病中系膜细胞的有用标志物。
