Chen Kang, Li Jie, Tang Jing, Zhao Fang-Gui, Liu Xin
School of Life Sciences, Laiyang Agricultural College, Qingdao 266109, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Oct;32(5):577-82.
Possible regulation of salt stress-induced ABA accumulation by nitric oxide (NO) in maize seedling was investigated. Both NO and ABA contents of maize leaves and root tips were increased in response to salt stress (Fig.1,2). Similar to the effects of salt stress, ABA contents of maize leaves and root tips were increased after the treatment of maize leaves with sodium nitroprusside (SNP, a nitric oxide donor) alone (Fig.3). Compared to the salt stress-induced ABA accumulation, this SNP-induced ABA increase was much faster, suggesting that NO may be an intermediate signal from salt stress to ABA accumulation. When NO production inhibitors L-NAME and NaN(3) treatments were applied, salt stress-induced ABA accumulation was lowered (Fig.4). Treatment with NO scavenger cPTIO also inhibited the salt stress-induced ABA increase (Fig.5). From these results it is deduced that NO is involved in regulation of ABA accumulation under salt stress.
研究了一氧化氮（NO）对盐胁迫诱导玉米幼苗脱落酸（ABA）积累的可能调节作用。盐胁迫下，玉米叶片和根尖的NO和ABA含量均增加（图1、2）。与盐胁迫的作用相似，单独用硝普钠（SNP，一种一氧化氮供体）处理玉米叶片后，玉米叶片和根尖的ABA含量增加（图3）。与盐胁迫诱导的ABA积累相比，这种SNP诱导的ABA增加要快得多，表明NO可能是从盐胁迫到ABA积累的中间信号。当应用NO产生抑制剂L-NAME和NaN₃处理时，盐胁迫诱导的ABA积累降低（图4）。用NO清除剂cPTIO处理也抑制了盐胁迫诱导的ABA增加（图5）。从这些结果可以推断，NO参与盐胁迫下ABA积累的调节。
