Haun M, Wasi S
Canadian Red Cross Society, National Reference Laboratory, Protein Chemistry Section, Ottawa, Ontario.
Anal Biochem. 1990 Dec;191(2):337-42. doi: 10.1016/0003-2697(90)90228-2.
Streptavidin was covalently bound to commercially available polyacrylamide beads (3-10 microns diameter) by peptide bond formation between the carboxyl groups on the solid matrix and the amino groups of the soluble protein. Biotinylated antibody or lectin was linked to the polyacrylamide beads via the streptavidin molecules. Immunoassays for human IgA1, IgA2, IgE, and vitronectin were developed utilizing the antibody or lectin as a capture ligand. The protein being assayed was quantitated colorimetrically at 492 nm via horseradish peroxidase-conjugated antibodies.
通过固体基质上的羧基与可溶性蛋白质的氨基之间形成肽键，将链霉亲和素共价结合到市售的聚丙烯酰胺珠（直径3 - 10微米）上。生物素化的抗体或凝集素通过链霉亲和素分子与聚丙烯酰胺珠相连。利用抗体或凝集素作为捕获配体，开发了针对人IgA1、IgA2、IgE和玻连蛋白的免疫测定方法。通过辣根过氧化物酶偶联抗体在492nm处比色法定量测定被测蛋白质。
