Hegstad R L, Johnston S D, Pasternak D M
Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Am J Vet Res. 1990 Dec;51(12):1941-7.
A commercially available radioimmunoassay (RIA) kit for measurement of human adrenocorticotropin (hACTH) was validated for use in dogs. Assay sensitivity was 3 pg/ml. Intra-assay coefficient of variation (x 100; CV) for 3 canine plasma pools was 3.0 (mean +/- SD, 33 +/- 0.99 pg/ml), 4.2 (71 +/- 2.4 pg/ml) and 3.7 (145 +/- 3.7 pg/ml) %. Interassay CV for 2 plasma pools measured in 6 assays was 9.8 (37 +/- 3.6 pg/ml) and 4.4 (76 +/- 3.4 pg/ml) %, respectively. Dilutional parallelism was documented by assaying 2 pools of canine plasma at 3 dilutions and correcting the measured result for dilution. Corrected mean concentrations for the first pool were 33 (+/- 0.99), 36 (+/- 4.3), and 33 (+/- 6.8) pg/ml; corrected mean concentrations for the second pool were 145 (+/- 5.4), 141 (+/- 10.8) and 125 (+/- 3.4) pg/ml. Recovery of 1-39hACTH added to canine plasma (6.25, 12.5, 25.0, 50.0, and 100.0 pg/ml) was linear and quantitative (slope = 0.890, R2 = 0.961). To test whether anticoagulant or the protease inhibitor, aprotinin, influences ACTH concentration in canine plasma, ACTH was measured in canine blood collected in 4 tubes containing anticoagulant: heparin (H), heparin + 500 kallikrein inhibitor units (KIU) of aprotinin/ml (HA), EDTA (E), and EDTA + aprotinin (EA). Plasma ACTH concentration was the same when samples containing H and HA, or HA and E were compared, and was significantly (P less than 0.01) lower in samples containing EA.(ABSTRACT TRUNCATED AT 250 WORDS)
一种用于测量人促肾上腺皮质激素（hACTH）的市售放射免疫分析（RIA）试剂盒经验证可用于犬类。该分析方法的灵敏度为3 pg/ml。3个犬血浆混合样本的批内变异系数（×100；CV）分别为3.0（均值±标准差，33±0.99 pg/ml）、4.2（71±2.4 pg/ml）和3.7（145±3.7 pg/ml）%。在6次分析中对2个血浆混合样本进行测量，其批间CV分别为9.8（37±3.6 pg/ml）和4.4（76±3.4 pg/ml）%。通过对2个犬血浆混合样本进行3种稀释度的分析，并对测量结果进行稀释校正，证明了稀释平行性。第一个混合样本的校正后平均浓度分别为33（±0.99）、36（±4.3）和33（±6.8）pg/ml；第二个混合样本的校正后平均浓度分别为145（±5.4）、141（±10.8）和125（±3.4）pg/ml。添加到犬血浆中的1-39hACTH（6.25、12.5、25.0、50.0和100.0 pg/ml）的回收率呈线性且定量（斜率 = 0.890，R2 = 0.961）。为了测试抗凝剂或蛋白酶抑制剂抑肽酶是否会影响犬血浆中的ACTH浓度，在装有以下抗凝剂的4支试管中采集犬血并测量ACTH：肝素（H）、肝素+每毫升500激肽释放酶抑制单位（KIU）的抑肽酶（HA）、乙二胺四乙酸（EDTA）以及EDTA+抑肽酶（EA）。当比较含H和HA或HA和E的样本时，血浆ACTH浓度相同，而含EA的样本中的ACTH浓度显著降低（P<0.01）。（摘要截短于250字）
