[川芎嗪、合成冰片对纤毛运动持续时间的影响]
[Effect of ligustrazine, synthetic borneol on lasting time of ciliary movement].
作者信息
Pan Jia, Zou Wen-Jun, Liu Jie, Zhang Bai-Jia, Liu Ya-Ou, Pan Xiao-Juan
机构信息
Department of Pharmacology, Sicuan Institute of Chinese Material Medica, Chengdu 610041, China.
出版信息
Zhong Yao Cai. 2006 Aug;29(8):813-6.
OBJECTIVE
To study the using of the method in vitro and in vivo of lasting time of ciliary movement in ligustrazine, synthetic borneol.
METHODS
(1) Method in vitro-The lasting time of ciliary movement was observed with forty-fold optical microscope after dropping liquid medicine on exteacorporeal frog palate mucosa. Then mucosa was cleared. It was observed whether the ciliary movement was recovered and the lasting time was recorded from recovering to stopping once again. (2) Method in vivo-Liquid medicine or normal saline was dropped on frog palate mucosa by contacting thirty minutes. Then mucosa was cleared. The lasting time of ciliary movement was observed with forty-fold optical microscope by separating palate mucosa.
RESULTS
Relatively percentage of lasting time of ciliary movement of ligustrazine in vitro and in vivo was 9.8%, 87.3%; The relatively percentage of synthetic borneol in vitro and in vivo was 9.3%, 89.5%.
CONCLUSION
The method in vitro and in vivo of lasting time of ciliary movement can be the one of the selecting ways of Chinese drug's toxicity of nasal mucosa, and it have virtues and defects respectively. Ligustrazine, synthetic borneol have significantly toxical effect on exteacorporeal lasting time of ciliary movement.
目的
研究体外及体内观察川芎嗪、合成冰片对纤毛运动持续时间的方法。
方法
（1）体外方法——将药液滴于体外蛙腭黏膜后，用40倍光学显微镜观察纤毛运动持续时间。然后清除黏膜。观察纤毛运动是否恢复，并记录从恢复到再次停止的持续时间。（2）体内方法——将药液或生理盐水滴于蛙腭黏膜上接触30分钟。然后清除黏膜。通过分离腭黏膜，用40倍光学显微镜观察纤毛运动持续时间。
结果
川芎嗪体外及体内纤毛运动持续时间相对百分比分别为9.8%、87.3%；合成冰片体外及体内相对百分比分别为9.3%、89.5%。
结论
体外及体内观察纤毛运动持续时间的方法可作为中药鼻黏膜毒性筛选方法之一，且各有优缺点。川芎嗪、合成冰片对体外纤毛运动持续时间有明显毒性作用。
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