Bao Jie, Zheng Lei, Wang Qian, Gu Chun-Yu, Qiu Yu-Rong, Li Juan
Laboratory Medicine Center, Nangfang Hospital, Southern Medical University, Guangzhou 510515, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Nov;22(6):703-5.
To explore the expression of avian reticuloendotheliosis viral(v-rel) oncogene-related B (RelB) mRNA in vitro in murine mature and immature myeloid dendritic cells (DCs).
The bone marrow was collected from the femur and tibias of C57BL/6 mice in sterile condition. Bone marrow precursors were cultured with recombinant mouse granulocyte-macrophage colony-stimulating factor (rmGM-CSF) and interleukin-4 (rmIL-4) to produce immature DCs. Immature DCs were stimulated by LPS 18 h before the end of culture to become mature. DCs' phenotype was detected by flow cytometry (FCM). RelB expression and protein level were determined by RT-PCR and immunofluorescence staining.
The expression level of co-stimulatory molecules (CD86 and CD40) and MHC-II class molecule were low in immature DCs, whereas high in mature DCs. RelB expression was significantly higher in mature DCs than in immature DCs (P<0.01).
RelB expression is closely associated with the maturative situation of DCs. Inhibition of RelB expression in DCs may induce tolerogenic DCs.
探讨禽网状内皮组织增生症病毒（v-rel）癌基因相关B（RelB）mRNA在小鼠成熟和未成熟髓样树突状细胞（DCs）中的体外表达情况。
无菌条件下从C57BL/6小鼠的股骨和胫骨采集骨髓。用重组小鼠粒细胞-巨噬细胞集落刺激因子（rmGM-CSF）和白细胞介素-4（rmIL-4）培养骨髓前体细胞以产生未成熟DCs。在培养结束前18小时用脂多糖刺激未成熟DCs使其成熟。通过流式细胞术（FCM）检测DCs的表型。通过RT-PCR和免疫荧光染色测定RelB的表达和蛋白水平。
未成熟DCs中共刺激分子（CD86和CD40）和MHC-II类分子的表达水平较低，而成熟DCs中表达水平较高。成熟DCs中RelB的表达明显高于未成熟DCs（P<0.01）。
RelB的表达与DCs的成熟状态密切相关。抑制DCs中RelB的表达可能诱导产生耐受性DCs。
