Zhang Yong-Qiang, Lu Xiao-He, Yuan Wei, Gong Yu-Bo, Zhou Jin, Peng Xiao-Juan
Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Nov;22(6):754-7.
To observe the apoptosis of cells in rat corneal grafts at acute rejection phase and explore the effects of IL-1 receptor antagonist (IL-1ra) on cell apoptosis.
The penetrating corneal transplantation model was established. Corneal grafting was divided into four groups, namely, normal Wistar rat control group (no grafting, group A), isograft (Wistar rat-->Wistar rat, group B), allograft (Wistar rat-->SD rat) with normal saline treatment (group C) and allograft (Wistar rat-->SD rat) with IL-1ra treatment (group D). Cell apoptosis in corneal grafts was detected by TUNEL staining at 7 d, 10 d and 14 d after transplantation, and an automatic image analyzer was used to analyze the results, which were expressed as positive unit (PU). The changes of cellular ultrastructure in corneal grafts were observed under transmission electron microscope.
(1)The average survival time of corneal grafts in C and D groups was (10.38+/-1.85) d and (13.56+/-1.94) d, respectively, with significant difference (P<0.01). (2)As compared with normal and non-rejected corneas, cell apoptosis and necrosis commonly existed in corneal grafts which rejection had occur. (3)In normal corneas, there were merely a very small number apoptotic cells in epithelial laminal, and apoptotic cells were found hardly in stromal laminal and endothelial cell layers. However, sporadic apoptotic cells were found in all layers of corneal grafts in B, C and D groups at 10 d after transplantation, the average PU being of no notably difference (P>0.05). Apoptosis obviously increased in nearby regions of wound and central area of corneal grafts in C and D groups, especially in C group. The apoptotic cells were distributed mainly in basal layer of epithelial cells and stroma of superficial layer.
Cell apoptosis plays an important role in corneal graft rejection reaction. IL-1ra treatment can prolong the survival time of corneal grafts by means of suppression of cell apoptosis in corneal grafts.
观察大鼠角膜移植急性排斥反应期移植片细胞凋亡情况，探讨白细胞介素-1受体拮抗剂（IL-1ra）对细胞凋亡的影响。
建立穿透性角膜移植模型。角膜移植分为四组，即正常Wistar大鼠对照组（未移植，A组）、同基因移植组（Wistar大鼠→Wistar大鼠，B组）、生理盐水处理的异体移植组（Wistar大鼠→SD大鼠，C组）和IL-1ra处理的异体移植组（Wistar大鼠→SD大鼠，D组）。于移植后7 d、10 d和14 d采用TUNEL染色检测角膜移植片中细胞凋亡情况，并用自动图像分析仪分析结果，以阳性单位（PU）表示。通过透射电子显微镜观察角膜移植片细胞超微结构的变化。
（1）C组和D组角膜移植片平均存活时间分别为（10.38±1.85）d和（13.56±1.94）d，差异有统计学意义（P<0.01）。（2）与正常及未发生排斥反应的角膜相比，发生排斥反应的角膜移植片中细胞凋亡和坏死普遍存在。（3）正常角膜上皮层仅有极少数凋亡细胞，基质层和内皮细胞层几乎未见凋亡细胞。而B、C、D组移植后10 d角膜移植片各层均可见散在凋亡细胞，平均PU差异无统计学意义（P>0.05）。C组和D组角膜移植片伤口附近区域及中央区凋亡明显增加，尤以C组为著。凋亡细胞主要分布于上皮细胞基底层和浅层基质。
细胞凋亡在角膜移植排斥反应中起重要作用。IL-1ra处理可通过抑制角膜移植片细胞凋亡延长角膜移植片存活时间。
