Koo Bon Chul, Kwon Mo Sun, Choi Bok Ryul, Kim Jin-Hoi, Cho Seong-Keun, Sohn Sea Hwan, Cho Eun Jung, Lee Hoon Taek, Chang Wonkyung, Jeon Iksoo, Park Jin-Ki, Park Jae Bok, Kim Teoan
Department of Physiology, Catholic University of Daegu School of Medicine, Daegu, Korea.
FASEB J. 2006 Nov;20(13):2251-60. doi: 10.1096/fj.06-5866com.
The Moloney murine leukemia virus (MoMLV) -based retrovirus vector system has been used most often in gene transfer work, but has been known to cause silencing of the imported gene in transgenic animals. In the present study, using a MoMLV-based retrovirus vector, we successfully generated a new transgenic chicken line expressing high levels of enhanced green fluorescent protein (eGFP). The level of eGFP expression was conserved after germline transmission and as much as 100 microg of eGFP could be detected per 1 mg of tissue protein. DNA sequencing showed that the transgene had been integrated at chromosome 26 of the G1 and G2 generation transgenic chickens. Owing to the stable integration of the transgene, it is now feasible to produce G3 generation of homozygous eGFP transgenic chickens that will provide 100% transgenic eggs. These results will help establish a useful transgenic chicken model system for studies of embryonic development and for efficient production of transgenic chickens as bioreactors.
基于莫洛尼鼠白血病病毒（MoMLV）的逆转录病毒载体系统在基因转移工作中使用最为频繁，但已知会导致转基因动物中导入基因的沉默。在本研究中，我们使用基于MoMLV的逆转录病毒载体，成功培育出一个新的转基因鸡品系，该品系能高水平表达增强型绿色荧光蛋白（eGFP）。在种系传递后，eGFP的表达水平得以保持，每1毫克组织蛋白中可检测到多达100微克的eGFP。DNA测序表明，转基因已整合到G1和G2代转基因鸡的26号染色体上。由于转基因的稳定整合，现在有可能培育出G3代纯合eGFP转基因鸡，这些鸡将产出100%的转基因鸡蛋。这些结果将有助于建立一个有用的转基因鸡模型系统，用于胚胎发育研究以及作为生物反应器高效生产转基因鸡。
