Naito M, Hayashi T, Funaki C, Kuzuya M, Asai K, Yamada K, Kuzuya F
Department of Geriatrics, Nagoya University School of Medicine, Japan.
Exp Cell Res. 1991 May;194(1):154-6. doi: 10.1016/0014-4827(91)90145-k.
Vitronectin, a multifunctional glycoprotein present in the plasma and interstitial tissues, has recently been found to be localized in atherosclerotic lesions. In this study we examined the effects of vitronectin on the migration of cultured bovine aortic smooth muscle cells using a modified Boyden chamber assay. The cells migrated to fluid-phase vitronectin in a concentration-dependent fashion. The cells also migrated to membrane filter surfaces precoated with vitronectin for a few minutes in the absence of additional vitronectin in the fluid phase, suggesting that this substance binds easily to the filters and stimulates cell migration by haptotaxis under the conditions described. These observations suggest that vitronectin deposited in the intima may be involved in the pathogenesis of atherosclerosis by recruiting smooth muscle cells from the media into the intima.
玻连蛋白是一种存在于血浆和间质组织中的多功能糖蛋白，最近被发现定位于动脉粥样硬化病变中。在本研究中，我们使用改良的博伊登室分析法检测了玻连蛋白对培养的牛主动脉平滑肌细胞迁移的影响。细胞以浓度依赖的方式向液相玻连蛋白迁移。在液相中不存在额外玻连蛋白的情况下，细胞也会迁移到预先用玻连蛋白包被几分钟的膜滤器表面，这表明该物质很容易与滤器结合，并在所述条件下通过趋触性刺激细胞迁移。这些观察结果表明，沉积在内膜中的玻连蛋白可能通过将中膜的平滑肌细胞募集到内膜而参与动脉粥样硬化的发病机制。
