Cilluffo M C, Cohen B N, Fain G L
Department of Ophthalmology, Jules Stein Eye Institute, UCLA School of Medicine 90024.
Invest Ophthalmol Vis Sci. 1991 Apr;32(5):1619-29.
The aqueous humor of the eye is thought to be secreted by the epithelium of the ciliary body. This epithelium has been difficult to study, in part because of its complicated morphology. The authors attempted to circumvent this difficulty by growing the epithelial cells in tissue culture. A procedure is described for producing pure primary cultures of rabbit nonpigmented ciliary body epithelial cells. This procedure was used with whole-cell patch-clamp recording to characterize voltage-activated currents in the nonpigmented cells. These experiments show that most nonpigmented cells contain two kinds of currents: a rapidly activating and inactivating inward current, carried by Na+ and blocked by tetrodotoxin (TTX), and a more slowly activating and inactivating outward current, blocked by tetraethylammonium (TEA+), Ba2+, and 4-aminopyridine (4-AP) and presumably carried by K+. Both of these currents have been observed in freshly dissociated cells and in cultures up to 7 days old. The voltage-gated currents in ciliary body epithelial cells are remarkably similar to those of neurons and raise the possibility that these epithelial cells are capable of spike propagation.
眼房水被认为是由睫状体上皮分泌的。这种上皮很难研究，部分原因是其形态复杂。作者试图通过在组织培养中培养上皮细胞来规避这一困难。本文描述了一种用于制备兔非色素睫状体上皮细胞纯原代培养物的方法。该方法与全细胞膜片钳记录一起用于表征非色素细胞中的电压激活电流。这些实验表明，大多数非色素细胞含有两种电流：一种是快速激活和失活的内向电流，由Na +携带并被河豚毒素（TTX）阻断；另一种是激活和失活较慢的外向电流，被四乙铵（TEA +）、Ba2 +和4-氨基吡啶（4-AP）阻断，可能由K +携带。在新鲜解离的细胞和培养7天以内的细胞中都观察到了这两种电流。睫状体上皮细胞中的电压门控电流与神经元的电流非常相似，这增加了这些上皮细胞能够进行动作电位传播的可能性。
