Bousfield George R, Butnev Vladimir Y, Walton Wendy J, Nguyen Van T, Huneidi Jennifer, Singh Vinod, Kolli V S Kumar, Harvey David J, Rance Naomi E
Wichita State University, Wichita, KS, United States.
Mol Cell Endocrinol. 2007 Jan 2;260-262:40-8. doi: 10.1016/j.mce.2006.02.017. Epub 2006 Oct 31.
Human FSH exists as two major glycoforms designated, tetra-glycosylated and di-glycosylated hFSH. The former possesses both alpha- and beta-subunit carbohydrates while the latter possesses only alpha-subunit carbohydrate. Western blotting differentiated the glycosylated, 24,000 M(r) hFSHbeta band from the non-glycosylated 21,000 M(r) FSHbeta band. Postmenopausal urinary hFSH preparations possessed 75-95% 24,000 M(r) hFSHbeta, while pituitary hFSH immunopurified from 21- to 43-year-old females and 21-43-year-old males possessed only 35-40% 24,000 M(r) hFSHbeta. The pituitary hFSH from a postmenopausal woman on estrogen replacement was 75% 21,000 M(r) hFSHbeta. Other immunopurified postmenopausal pituitary hFSH preparations possessed 50-60% 21,000 M(r) hFSHbeta. Gel filtration removed predominantly 21,000 M(r) free hFSHbeta and reduced its abundance to 13-22% in postmenopausal pituitary hFSH heterodimer preparations. A major regulatory mechanism for FSH glycosylation involves control of beta-subunit N-glycosylation, possibly by inhibition of oligosaccharyl transferase. Two primate species exhibited the same all-or-none pattern of pituitary FSHbeta glycosylation.
人促卵泡激素（FSH）以两种主要糖型存在，分别称为四糖基化和二糖基化的hFSH。前者同时具有α亚基和β亚基碳水化合物，而后者仅具有α亚基碳水化合物。蛋白质印迹法区分了糖基化的24,000 M（r）hFSHβ条带和非糖基化的21,000 M（r）FSHβ条带。绝经后尿hFSH制剂含有75 - 95%的24,000 M（r）hFSHβ，而从21至43岁女性和21 - 43岁男性中免疫纯化的垂体hFSH仅含有35 - 40%的24,000 M（r）hFSHβ。接受雌激素替代治疗的绝经后女性的垂体hFSH中75%为21,000 M（r）hFSHβ。其他免疫纯化的绝经后垂体hFSH制剂含有50 - 60%的21,000 M（r）hFSHβ。凝胶过滤主要去除了21,000 M（r）的游离hFSHβ，并使绝经后垂体hFSH异二聚体制剂中其丰度降低至13 - 22%。FSH糖基化的一种主要调节机制可能涉及对β亚基N - 糖基化的控制，可能是通过抑制寡糖基转移酶。两种灵长类动物表现出相同的垂体FSHβ糖基化全有或全无模式。
