Yamaguchi Yoshifumi, Yonemura Shigenobu, Takada Shinji
Okazaki Institute for Integrative Biosciences, National Institutes of Natural Sciences, Myodaiji, Okazaki, 444-8787, Japan.
Development. 2006 Dec;133(23):4737-48. doi: 10.1242/dev.02658. Epub 2006 Nov 1.
Duct epithelial structure is an essential feature of many internal organs, including exocrine glands and the kidney. The ducts not only mediate fluid transfer but also help to maintain homeostasis. For instance, fluids and solutes are resorbed from or secreted into the primary fluid flowing through the lumen of the ducts in the exocrine glands and kidneys. The molecular mechanism underlying the functional maturation of these ducts remains largely unknown. Here, we show that a grainyhead-related transcription factor, CP2-like 1 (CP2L1), is required for the maturation of the ducts of the salivary gland and kidney. In the mouse, Cp2l1 is specifically expressed in the developing ducts of a number of exocrine glands, including the salivary gland, as well as in those of the kidney. In Cp2l1-deficient mice, the expression of genes directly involved in functional maturation of the ducts was specifically reduced in both the salivary gland and kidney, indicating that Cp2l1 is required for the differentiation of duct cells. Furthermore, the composition of saliva and urine was abnormal in these mice. These results indicate that Cp2l1 expression is required for normal duct development in both the salivary gland and kidney.
导管上皮结构是许多内脏器官的基本特征，包括外分泌腺和肾脏。导管不仅介导液体转运，还有助于维持体内平衡。例如，在流经外分泌腺和肾脏导管管腔的初级液体中，液体和溶质会被重吸收或分泌。这些导管功能成熟的分子机制仍 largely 未知。在这里，我们表明一种与颗粒头相关的转录因子，即 CP2 样 1（CP2L1），是唾液腺和肾脏导管成熟所必需的。在小鼠中，Cp2l1 在包括唾液腺在内的一些外分泌腺以及肾脏的发育中的导管中特异性表达。在 Cp2l1 缺陷小鼠中，唾液腺和肾脏中直接参与导管功能成熟的基因表达均特异性降低，表明 Cp2l1 是导管细胞分化所必需的。此外，这些小鼠的唾液和尿液成分异常。这些结果表明，Cp2l1 的表达是唾液腺和肾脏正常导管发育所必需的。
