体外试验表明，在一名接受齐多夫定（ZDV）长期治疗的患者的两株HIV-1分离株中，逆转录酶活性与核心抗原（p24）产生出现解离。

In vitro assays show a dissociation of reverse transcriptase activity and core antigen (p24) production in two HIV-1 isolates from a patient receiving long-term treatment with zidovudine (ZDV).

作者信息

Masquelier B, Combeau T, Poveda J D, Delord B, Pellegrin J L, Sallafranque-Andreola M L, Tarrago-Litvak L, Fleury H J

机构信息

Laboratoire de Virologie, Université de Bordeaux II, France.

出版信息

J Acquir Immune Defic Syndr (1988). 1991;4(5):499-505.

PMID:1707962

Abstract

Two HIV-1 isolates were obtained from a patient receiving long-term treatment with zidovudine (ZDV). The in vitro sensitivity to ZDV triphosphate of the reverse transcriptase (RT) from both isolates appeared to be unchanged compared to that of the LAV-Bru HIV-1 reference strain. When isolates were grown in CEM cells (a T-lymphoblastoid tumor cell line) and their RT activity and core antigen (p24) production were determined, the level of p24 production compared to RT activity was high; in infected CEM cells treated with ZDV, RT activity was at background level while the p24 production was still significant, thus indicating a dissociation of RT activity and core antigen production.

摘要

从一名接受齐多夫定（ZDV）长期治疗的患者身上获得了两株HIV-1分离株。与LAV-Bru HIV-1参考菌株相比，这两株分离株的逆转录酶（RT）对三磷酸齐多夫定的体外敏感性似乎没有变化。当分离株在CEM细胞（一种T淋巴细胞样肿瘤细胞系）中生长并测定其RT活性和核心抗原（p24）产量时，与RT活性相比，p24产量水平较高；在用ZDV处理的受感染CEM细胞中，RT活性处于背景水平，而p24产量仍然很高，因此表明RT活性与核心抗原产生解离。