Al-Jahdari Wael S, Saito Shigeru, Nakano Takashi, Goto Fumio
Department of Anesthesiology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi-city, Gunma, 371-8511, Japan.
Can J Anaesth. 2006 Nov;53(11):1078-85.
Propofol neurotoxicity has been demonstrated in several cell culture systems. This study was undertaken to determine whether propofol has neurotoxic effects on peripheral, retinal, and autonomic neurons, and which neurons are particularly liable to injury by propofol.
Dorsal root ganglia, retinal ganglion cell layers, and sympathetic ganglion chains were isolated from day eight chick embryos and cultured for 20 hr. Thereafter, propofol was added at various concentrations [5-300 microM (0.9-53 microg x mL(-1))] to investigate its effects on these three types of neuronal tissue. Morphological changes were examined quantitatively by growth cone collapse assay. Propofol concentrations were measured using high performance liquid chromatography.
Propofol induced growth cone collapse and neurite destruction. The three types of neurons tested exhibited significantly different dose-response relationships two hours after the application of propofol (P < 0.001) but not at 24 hr after application. The growth cone-collapsing effect was at least partially reversible in all three types of neurons after exposure to 100 microM propofol up to six hours, though reversibility was not observed after 24-hr exposure.
While the clinical safety profile of propofol has been well documented, at high concentrations propofol has potential neurotoxicity on growing neurons in vitro.
丙泊酚的神经毒性已在多种细胞培养系统中得到证实。本研究旨在确定丙泊酚是否对周围神经、视网膜神经和自主神经元具有神经毒性作用，以及哪些神经元特别容易受到丙泊酚的损伤。
从第8天的鸡胚中分离出背根神经节、视网膜神经节细胞层和交感神经节链，并培养20小时。此后，添加不同浓度的丙泊酚[5 - 300微摩尔/升(0.9 - 53微克/毫升)]，以研究其对这三种神经元组织的影响。通过生长锥塌陷试验定量检测形态学变化。使用高效液相色谱法测量丙泊酚浓度。
丙泊酚诱导生长锥塌陷和神经突破坏。在应用丙泊酚两小时后，所测试的三种神经元表现出显著不同的剂量反应关系(P < 0.001)，但在应用24小时后未观察到差异。在暴露于100微摩尔/升丙泊酚长达6小时后，所有三种神经元的生长锥塌陷效应至少部分可逆，尽管在暴露24小时后未观察到可逆性。
虽然丙泊酚的临床安全性已得到充分证明，但在高浓度下，丙泊酚在体外对生长中的神经元具有潜在的神经毒性。
