Hruska Vlastimil, Stedrý Milan, Vceláková Katerina, Lokajová Jana, Tesarová Eva, Jaros Michal, Gas Bohuslav
Faculty of Science, Charles University, Prague, Czech Republic.
Electrophoresis. 2006 Dec;27(23):4610-7. doi: 10.1002/elps.200600277.
We present a mathematical model of CZE based on the concept of eigenmobilities - the eigenvalues of matrix M tied to the linearized governing equations of electromigration, and the spectral decomposition of matrix M into matrices of amplitudes P(j). Any peak in an electropherogram, regardless of whether it is an analyte peak or a system peak (system zone), is matched with its matrix P(j). This enables calculation of the peak parameters, such as the transfer ratio and the molar conductivity detection response (which give the indirect detection signal and the conductivity detection signal, respectively), when the initial disturbance caused by the injection of the sample is known. We also introduce new quantities, such as the generalized transfer ratio and the conductivity response of system zones, and show how the amplitude (intensity, area) of the analyte peaks and the system peaks can be calculated. We offer a free software, PeakMaster (http://www.natur.cuni.cz/gas), which yields this information in a user-friendly way.
我们基于本征迁移率的概念提出了一种毛细管区带电泳（CZE）的数学模型——矩阵M的特征值与电迁移线性化控制方程相关联，以及矩阵M的谱分解为振幅矩阵P(j)。电泳图中的任何峰，无论它是分析物峰还是系统峰（系统区带），都与其矩阵P(j)相匹配。当已知由样品注入引起的初始扰动时，这使得能够计算峰参数，例如转移比和摩尔电导率检测响应（分别给出间接检测信号和电导率检测信号）。我们还引入了新的量，例如广义转移比和系统区带的电导率响应，并展示了如何计算分析物峰和系统峰的振幅（强度、面积）。我们提供了一个免费软件PeakMaster（http://www.natur.cuni.cz/gas），它以用户友好的方式提供这些信息。
