Kałuzewski S, Jagielski M, Zaleska M, Szych J
Zakład Bakteriologii PZH w Warszawie.
Med Dosw Mikrobiol. 1990;42(1-2):26-34.
The aim of this study was to evaluate a latex reagent prepared in our laboratory for a routine diagnosis of Salmonellosis in humans. Liquid cultures in selenite broth (SF) (18-24 hr), previously inoculated with faeces samples of individuals suspected of being infected with Salmonella were subjected to the study. In these cultures, after 15 min. of heating at boiling temperatures, group antigens of Salmonella with an aid of polyvalent latex reagent A-E and monovalent reagents B, C1, C2, D, and E were searched. The results of latex test were compared to the results obtained by routine bacteriological examination. Studies performed in 13 laboratories of Sanitary Epidemiological Stations included 5246 faeces samples. Out of these samples 1835 (35%) reacted with monovalent latex reagent and 1897 (36.2%) samples were positive, for Salmonella by culture technique and belonged to 14 genera of group B, C1, D, and E. S. enteritidis was the most frequently isolated and encountered for 98.6% of all isolated strains. Latex test with A-E reagent was positive in 2246 (42.8%) of culture samples in SF medium, of which 1736 were positive by culture and 510 samples were negative for Salmonella in routine bacteriological examination. The samples positive in culture and with A-E latex reagent reacted in 97.2% with one monovalent reagent. Out of bacteriologically negative samples and reacting with A-E latex reagent 28.8% were positive with monovalent latex reagents. In summary, we can conclude that latex test used in a survey studies can be an usefull test in addition to routine bacteriological examination, since after 18-24 hr it allows with high credibility of 95% to confirm or exclude Salmonella in a tested sample. Such a procedure due to a shortening of routine diagnostic course brings significant savings. Moreover, latex test makes possible rapid detection of mixed infections with Salmonella of different serological groups. The use of extremely carefully, properly prepared selenite broth constitutes a basic condition for agreement between results of latex test and routine bacteriological investigation.
本研究的目的是评估我们实验室制备的一种乳胶试剂，用于人类沙门氏菌病的常规诊断。对先前接种了疑似感染沙门氏菌个体粪便样本的亚硒酸盐肉汤（SF）液体培养物（18 - 24小时）进行研究。在这些培养物中，在沸腾温度下加热15分钟后，借助多价乳胶试剂A - E和单价试剂B、C1、C2、D和E搜索沙门氏菌的群抗原。将乳胶试验结果与常规细菌学检查获得的结果进行比较。在13个卫生流行病学站实验室进行的研究包括5246份粪便样本。在这些样本中，1835份（35%）与单价乳胶试剂反应，1897份（36.2%）样本通过培养技术检测出沙门氏菌呈阳性，属于B、C1、D和E组的14个属。肠炎沙门氏菌是最常分离到的，占所有分离菌株的98.6%。在SF培养基中的培养样本中，用A - E试剂进行乳胶试验，2246份（42.8%）呈阳性，其中1736份通过培养呈阳性，510份样本在常规细菌学检查中沙门氏菌呈阴性。培养阳性且与A - E乳胶试剂反应的样本与一种单价试剂的反应率为97.2%。在细菌学检查阴性且与A - E乳胶试剂反应的样本中，28.8%与单价乳胶试剂呈阳性反应。总之，我们可以得出结论，在调查研究中使用的乳胶试验除常规细菌学检查外可能是一项有用的检测，因为在18 - 24小时后，它能以95%的高可信度确认或排除检测样本中的沙门氏菌。由于缩短了常规诊断过程，这样的程序带来了显著的节省。此外，乳胶试验使得能够快速检测不同血清群沙门氏菌的混合感染。极其小心、正确制备亚硒酸盐肉汤的使用是乳胶试验结果与常规细菌学调查结果一致的基本条件。
