Okamoto T, Sumikawa K
Department of Psychobiology, University of California, Irvine 92717.
Brain Res Mol Brain Res. 1991 Jan;9(1-2):165-8. doi: 10.1016/0169-328x(91)90144-m.
ACh receptors (AChRs) synthesized in Xenopus oocytes which were cultured in medium containing gentamicin desensitized much more rapidly than those expressed in the absence of gentamicin. The effect caused by 24 h incubation in gentamicin could not be reversed by leaving oocytes in culture medium without gentamicin for 24 h. In addition, gentamicin exhibited a direct reversible blocking action on the function of AChRs. Our experiments indicate that antibiotics should be used cautiously in culturing oocytes when studying the function of induced neurotransmitter receptors and ion channels.
在含有庆大霉素的培养基中培养的非洲爪蟾卵母细胞合成的乙酰胆碱受体（AChRs）比在无庆大霉素条件下表达的受体脱敏速度快得多。在庆大霉素中孵育24小时所产生的效应，不能通过将卵母细胞置于不含庆大霉素的培养基中培养24小时来逆转。此外，庆大霉素对AChRs的功能表现出直接的可逆性阻断作用。我们的实验表明，在研究诱导型神经递质受体和离子通道的功能时，在培养卵母细胞时应谨慎使用抗生素。
