Kiyama H, McGowan E M, Emson P C
MRC Group, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge.
Brain Res Mol Brain Res. 1991 Jan;9(1-2):87-93. doi: 10.1016/0169-328x(91)90133-i.
Improvements in the sensitivity of non-radioactive in situ hybridization histochemistry methods for detection of mRNA now make it feasible to combine the use of non-radioactive and radioactive in situ methods to visualize two mRNAs on the same tissue section. The method reported here allows the simultaneous detection of two mRNAs in one cell and therefore is ideally suited to the studies of co-expression. Here we demonstrate the co-expression of tyrosine hydroxylase (TH) mRNA and cholecystokinin (CCK) mRNA in the ventral mesencephalic dopaminergic neurones of the rat. The distribution of dopaminergic neurones containing both TH and CCK transcripts suggests, on the basis of earlier anatomical studies that these CCK/TH-containing doubled-labelled cells project mainly to the striatal matrix. Dopamine neurones believed to project to the patch compartment did not contain CCK mRNA.
非放射性原位杂交组织化学方法检测mRNA的灵敏度提高，使得将非放射性和放射性原位方法结合用于在同一组织切片上可视化两种mRNA成为可能。本文报道的方法能够在单个细胞中同时检测两种mRNA，因此非常适合共表达研究。在此，我们证明了大鼠腹侧中脑多巴胺能神经元中酪氨酸羟化酶（TH）mRNA和胆囊收缩素（CCK）mRNA的共表达。根据早期的解剖学研究，同时含有TH和CCK转录本的多巴胺能神经元的分布表明，这些含CCK/TH的双标记细胞主要投射到纹状体基质。据信投射到纹状体小片区的多巴胺能神经元不含CCK mRNA。
