Jonsson K Hanna M, Weintraub Andrej, Widmalm Göran
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S-106 91 Stockholm, Sweden.
Carbohydr Res. 2006 Dec 29;341(18):2986-9. doi: 10.1016/j.carres.2006.10.005. Epub 2006 Oct 10.
The structures of the O-antigenic part of the lipopolysaccharides from Shigella dysenteriae type 3 and Escherichia coli O124 have been reinvestigated. (1)H and (13)C NMR spectroscopy in combination with selected 2D NMR techniques were used to determine the O-antigen pentasaccharide repeating units with the following structure: [see text]. From biosynthetic considerations this should also be the biological repeating unit. The structures of the repeating units also explain the previously observed cross-reactivity between the strains and to E. coli O164, which only differs in the terminal sugar residue that is lacking the (R)-1-carboxyethyl group.
对痢疾志贺氏菌3型和大肠杆菌O124脂多糖的O抗原部分结构进行了重新研究。使用¹H和¹³C NMR光谱结合选定的二维NMR技术来确定具有以下结构的O抗原五糖重复单元：[见原文]。从生物合成的角度考虑，这也应该是生物学重复单元。重复单元的结构还解释了之前观察到的这些菌株与大肠杆菌O164之间的交叉反应性，大肠杆菌O164仅在末端糖残基上有所不同，该末端糖残基缺少（R）-1-羧乙基基团。
