Tassi V, Ruggiero G, Rosaia L, Lorenzoni P, Ceccarini C, Tecce M F
Sclavo Research Center, Siena, Italy.
Immunol Lett. 1991 Jan;27(1):39-43. doi: 10.1016/0165-2478(91)90241-2.
Within the idiotype/anti-idiotype network, immunoglobulins act alternatively as reactive molecules and as antigens. To investigate the antigenic properties of immunoglobulins, we evaluated the immunogenicity in rabbits of three murine monoclonal anti-idiotypic antibodies and of their F(ab')2 fragments. These antibodies, bearing the internal image of a human melanoma antigen, may be useful in view of a human therapeutic application. The effect was evaluated as specific anti-anti-idiotypic response, related to the immunogenicity of the idiotypic epitopes in the combining sites of the immunoglobulins, and as total anti-murine immunoglobulin response, which represents the recognition of all the immunological determinants of the molecule. The results showed that the administration of the F(ab')2 fragments results in either higher or similar degrees of anti-anti-idiotypic immunization, compared to those induced by the whole immunoglobulins. Nevertheless, when anti-anti-idiotypic immunogenicity was increased, the anti-murine response did not increase proportionally. This suggests that the use for in vivo administration of F(ab')2 fragments is more convenient than the use of their original molecules, since this results, at least, in a similar or eventually in an increased specific immunogenicity, while the possibility of aspecific recognition is reduced.
在独特型/抗独特型网络中，免疫球蛋白可交替作为反应性分子和抗原发挥作用。为了研究免疫球蛋白的抗原特性，我们评估了三种鼠源单克隆抗独特型抗体及其F(ab')2片段在兔体内的免疫原性。这些抗体具有人黑色素瘤抗原的内影像，鉴于其在人类治疗中的应用前景，可能具有一定价值。评估的效应包括与免疫球蛋白结合位点中独特型表位免疫原性相关的特异性抗抗独特型反应，以及代表对该分子所有免疫决定簇识别的总抗鼠免疫球蛋白反应。结果表明，与完整免疫球蛋白诱导的反应相比，F(ab')2片段的给药导致了更高或相似程度的抗抗独特型免疫。然而，当抗抗独特型免疫原性增加时，抗鼠反应并未成比例增加。这表明，F(ab')2片段用于体内给药比使用其原始分子更方便，因为这至少会导致相似或最终增加的特异性免疫原性，同时减少了非特异性识别的可能性。
