Ikema Yasunari, Tohyama Harukazu, Yamamoto Ei, Kanaya Fuminori, Yasuda Kazunori
Department of Sports Medicine and Joint Reconstruction Surgery, Hokkaido University School of Medicine, Kita-15 Nishi-7, Sapporo 060-8638, Japan.
Clin Biomech (Bristol). 2007 Jan;22(1):120-6. doi: 10.1016/j.clinbiomech.2006.08.010. Epub 2006 Nov 2.
After ligament reconstruction, mechanical deterioration of the grafted tendon is observed with revascularization and cellular infiltration. However, the effect of cellular infiltration on the mechanical properties of the tendon matrix has not been fully understood.
Cultured fibroblasts derived from the rabbit patellar tendon were seeded around an acellular rabbit patellar tendon that had undergone freeze-thaw treatment. At time-0, 3, and 6 weeks after seeding the cells, we evaluated cellular distribution in the tendon using a confocal laser microscope and the mechanical evaluations of the tendon fascicles and the tendon bundles.
The confocal laser microscopic analysis showed fibroblast infiltration ex vivo into the acellular tendon matrix. We could not find significant effects of the cellular infiltration on the tangent modulus of the tendon bundle, although the ex vivo cellular infiltration significantly reduced the modulus of the tendon fascicle. In addition, the tangent modulus of the incubated tendon without fibroblasts significantly decreased with time, particularly in the tendon bundle levels.
The findings of this study suggested that the effects of ex vivo cellular infiltration on the mechanical properties of the tendon bundles are relatively small, compared with its striking effect on the tendon fascicles.
韧带重建后，随着血管再生和细胞浸润，移植肌腱会出现机械性能退化。然而，细胞浸润对肌腱基质机械性能的影响尚未完全明确。
将源自兔髌腱的培养成纤维细胞接种于经过冻融处理的脱细胞兔髌腱周围。在接种细胞后的0周、3周和6周，我们使用共聚焦激光显微镜评估细胞在肌腱中的分布情况，并对肌腱束和肌腱纤维束进行力学评估。
共聚焦激光显微镜分析显示，成纤维细胞在体外浸润到脱细胞肌腱基质中。尽管体外细胞浸润显著降低了肌腱纤维束的模量，但我们未发现细胞浸润对肌腱束的切线模量有显著影响。此外，未接种成纤维细胞的孵育肌腱的切线模量随时间显著降低，尤其是在肌腱束水平。
本研究结果表明，与细胞浸润对肌腱纤维束的显著影响相比，其对肌腱束机械性能的影响相对较小。
