用于空肠弯曲菌转录分析的八种RNA分离方法的评估
Evaluation of eight RNA isolation methods for transcriptional analysis in Campylobacter jejuni.
作者信息
Phongsisay Vongsavanh, Perera Viraj N, Fry Benjamin N
机构信息
School of Applied Sciences, Royal Melbourne Institute of Technology University, Melbourne, Budoora, 3083, Victoria, Australia.
出版信息
J Microbiol Methods. 2007 Feb;68(2):427-9. doi: 10.1016/j.mimet.2006.09.002. Epub 2006 Nov 3.
PMID:17084472
Abstract
DNA-free RNA samples are essential to investigate gene regulation using real-time RT-PCR. This study evaluated eight RNA isolation methods in combination with TURBO DNase treatment and acid phenol extractions for their ability to produce DNA-free RNA from Campylobacter jejuni strains.
摘要
无DNA的RNA样本对于使用实时逆转录聚合酶链反应（RT-PCR）研究基因调控至关重要。本研究评估了八种RNA提取方法，并结合TURBO脱氧核糖核酸酶（DNase）处理和酸性酚抽提，以考察它们从空肠弯曲杆菌菌株中产生无DNA RNA的能力。
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